ArticlePloS one2014
Maintenance and neuronal cell differentiation of neural stem cells C17.2 correlated to medium availability sets design criteria in microfluidic systems.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- CD271 Identifies a Subpopulation with Enhanced Neural-like Potential Within Wharton Jelly Derived Mesenchymal Stem/Stromal Cells.International journal of molecular sciences · 2026Article
- On-Chip Neural Induction Boosts Neural Stem Cell Commitment: Toward a Pipeline for iPSC-Based Therapies.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Hyaluronic acid-based bioink improves the differentiation and network formation of neural progenitor cells.Frontiers in bioengineering and biotechnology · 2023Article
- Microfluidics for Neuronal Cell and Circuit Engineering.Chemical reviews · 2022Review
- Three dimensional and microphysiological bone marrow models detect in vivo positive compounds.Scientific reports · 2021Article
- Microfluidics as efficient technology for the isolation and characterization of stem cells.EXCLI journal · 2021Review
- Nanobiohybrids: Materials approaches for bioaugmentation.Science advances · 2020Review
- Microfluidic Brain-on-a-Chip: Perspectives for Mimicking Neural System Disorders.Molecular neurobiology · 2019Review
- Prospect and challenge of detecting dynamic gene copy number increases in stem cells by whole genome sequencing.Journal of molecular medicine (Berlin, Germany) · 2019Article
- The RNA-Binding Protein RBM3 Promotes Neural Stem Cell (NSC) Proliferation Under Hypoxia.Frontiers in cell and developmental biology · 2019Article
- Stem cell culture and differentiation in microfluidic devices toward organ-on-a-chip.Future science OA · 2017Review
- Microfluidic engineering of neural stem cell niches for fate determination.Biomicrofluidics · 2017Article
- Microfluidic systems for stem cell-based neural tissue engineering.Lab on a chip · 2016Review
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Authors and funding
3 authors.
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Abstract
backgroundNeural stem cells (NSCs) play an important role in developing potential cell-based therapeutics for neurodegenerative disease. Microfluidics has proven a powerful tool in mechanistic studies of NSC differentiation. However, NSCs are prone to differentiate when the nutrients are limited, which occurs unfavorable by fast medium consumption in miniaturized culture environment. For mechanistic studies of NSCs in microfluidics, it is vital that neuronal cell differentiation is triggered by controlled factors only. Thus, we studied the correlation between available cell medium and spontaneous neuronal cell differentiation of C17.2 NSCs in standard culture medium, and proposed the necessary microfluidic design criteria to prevent undesirable cell phenotype changes. METHODOLOGY/PRINCIPAL
findingsA series of microchannels with specific geometric parameters were designed to provide different amount of medium to the cells over time. A medium factor (MF, defined as the volume of stem cell culture medium divided by total number of cells at seeding and number of hours between medium replacement) successfully correlated the amount of medium available to each cell averaged over time to neuronal cell differentiation. MF smaller than 8.3×10(4) µm3/cell⋅hour produced significant neuronal cell differentiation marked by cell morphological change and significantly more cells with positive β-tubulin-III and MAP2 staining than the control. When MF was equal or greater than 8.3×10(4) µm3/cell⋅hour, minimal spontaneous neuronal cell differentiation happened relative to the control. MF had minimal relation with the average neurite length. SIGNIFICANCE: MFs can be controlled easily to maintain the stem cell status of C17.2 NSCs or to induce spontaneous neuronal cell differentiation in standard stem cell culture medium. This finding is useful in designing microfluidic culture platforms for controllable NSC maintenance and differentiation. This study also offers insight about consumption rate of serum molecules involved in maintaining the stemness of NSCs.
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