Evidence map›Paper›PMID 25310299›Full record

ArticlePloS one2014

The structure of an LIM-only protein 4 (LMO4) and Deformed epidermal autoregulatory factor-1 (DEAF1) complex reveals a common mode of binding to LMO4.

Soumya Joseph, Ann H Kwan, Philippa H Stokes, Joel P Mackay, Liza Cubeddu, Jacqueline M Matthews

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.9field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 14 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Soumya JosephSchool of Molecular Bioscience, University of Sydney, Sydney, NSW, Australia.
Ann H KwanSchool of Molecular Bioscience, University of Sydney, Sydney, NSW, Australia.
Philippa H StokesSchool of Molecular Bioscience, University of Sydney, Sydney, NSW, Australia.
Joel P MackaySchool of Molecular Bioscience, University of Sydney, Sydney, NSW, Australia.
Liza CubedduSchool of Molecular Bioscience, University of Sydney, Sydney, NSW, Australia; School of Science and Health, University of Western Sydney, Campbelltown, NSW Australia.
Jacqueline M MatthewsSchool of Molecular Bioscience, University of Sydney, Sydney, NSW, Australia.
University of Sydney · AUWestern Sydney University · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

LIM-domain only protein 4 (LMO4) is a widely expressed protein with important roles in embryonic development and breast cancer. It has been reported to bind many partners, including the transcription factor Deformed epidermal autoregulatory factor-1 (DEAF1), with which LMO4 shares many biological parallels. We used yeast two-hybrid assays to show that DEAF1 binds both LIM domains of LMO4 and that DEAF1 binds the same face on LMO4 as two other LMO4-binding partners, namely LIM domain binding protein 1 (LDB1) and C-terminal binding protein interacting protein (CtIP/RBBP8). Mutagenic screening analysed by the same method, indicates that the key residues in the interaction lie in LMO4LIM2 and the N-terminal half of the LMO4-binding domain in DEAF1. We generated a stable LMO4LIM2-DEAF1 complex and determined the solution structure of that complex. Although the LMO4-binding domain from DEAF1 is intrinsically disordered, it becomes structured on binding. The structure confirms that LDB1, CtIP and DEAF1 all bind to the same face on LMO4. LMO4 appears to form a hub in protein-protein interaction networks, linking numerous pathways within cells. Competitive binding for LMO4 therefore most likely provides a level of regulation between those different pathways.

Indexed as

Adaptor Proteins, Signal TransducingDNA-Binding ProteinsHumansLIM Domain ProteinsNuclear ProteinsProtein BindingProtein Structure, TertiaryTranscription FactorsTwo-Hybrid System TechniquesAdaptor Proteins, Signal TransducingDEAF1 protein, humanDNA-Binding ProteinsLIM Domain ProteinsLMO4 protein, humanNuclear ProteinsTranscription Factors

Identifiers

PMID25310299
PMCPMC4195752
OpenAlexW2095266053

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.