ArticleeLife2014
A structural mechanism for bacterial autotransporter glycosylation by a dodecameric heptosyltransferase family.
Article in eLife, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 36 citations in OpenAlex.
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- NMR Chemical Shift Prediction of Glycopeptides and Glycoproteins Aided by the Computer Program CASPER.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Genetic variation, structural analysis, and virulence implications of BimA and BimC in clinical isolates of Burkholderia pseudomallei in Thailand.Scientific reports · 2024Article
- Dual function of the O-antigen WaaL ligase of Aggregatibacter actinomycetemcomitans.Molecular oral microbiology · 2023Article
- Insights into the biosynthesis of septacidin l-heptosamine moiety unveils a VOC family sugar epimerase.Acta pharmaceutica Sinica. B · 2023Article
- Folding Control in the Path of Type 5 Secretion.Toxins · 2021Review
- Conserved Conformational Hierarchy across Functionally Divergent Glycosyltransferases of the GT-B Structural Superfamily as Determined from Microsecond Molecular Dynamics.International journal of molecular sciences · 2021Article
- Crystal structures of β-1,4-N-acetylglucosaminyltransferase 2: structural basis for inherited muscular dystrophies.Acta crystallographica. Section D, Structural biology · 2021Article
- Semiprocessive Hyperglycosylation of Adhesin by Bacterial ProteinACS chemical biology · 2021Article
- Synthetic Glycobiology: Parts, Systems, and Applications.ACS synthetic biology · 2020Review
- Processivity in Bacterial Glycosyltransferases.ACS chemical biology · 2020Review
- Fusion of DARPin to Aldolase Enables Visualization of Small Protein by Cryo-EM.Structure (London, England : 1993) · 2019Article
- Article
- Insights into bacterial protein glycosylation in human microbiota.Science China. Life sciences · 2016Review
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 2 countries.
Funding
Abstract
A large group of bacterial virulence autotransporters including AIDA-I from diffusely adhering E. coli (DAEC) and TibA from enterotoxigenic E. coli (ETEC) require hyperglycosylation for functioning. Here we demonstrate that TibC from ETEC harbors a heptosyltransferase activity on TibA and AIDA-I, defining a large family of bacterial autotransporter heptosyltransferases (BAHTs). The crystal structure of TibC reveals a characteristic ring-shape dodecamer. The protomer features an N-terminal β-barrel, a catalytic domain, a β-hairpin thumb, and a unique iron-finger motif. The iron-finger motif contributes to back-to-back dimerization; six dimers form the ring through β-hairpin thumb-mediated hand-in-hand contact. The structure of ADP-D-glycero-β-D-manno-heptose (ADP-D,D-heptose)-bound TibC reveals a sugar transfer mechanism and also the ligand stereoselectivity determinant. Electron-cryomicroscopy analyses uncover a TibC-TibA dodecamer/hexamer assembly with two enzyme molecules binding to one TibA substrate. The complex structure also highlights a high efficient hyperglycosylation of six autotransporter substrates simultaneously by the dodecamer enzyme complex.
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Registered trials
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