Evidence map›Paper›PMID 25310188›Full record

ArticlePloS one2014

Action potential-evoked calcium release is impaired in single skeletal muscle fibers from heart failure patients.

Marino DiFranco, Marbella Quiñonez, Perry Shieh, Gregg C Fonarow, Daniel Cruz, Mario C Deng, Julio L Vergara, Holly R Middlekauff

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.6field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 5 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Marino DiFrancoDepartment of Physiology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, United States of America.
Marbella QuiñonezDepartment of Physiology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, United States of America.
Perry ShiehDepartment of Neurology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, United States of America.
Gregg C FonarowDivision of Cardiology, Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, United States of America.
Daniel CruzDivision of Cardiology, Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, United States of America.
Mario C DengDivision of Cardiology, Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, United States of America.
Julio L VergaraDepartment of Physiology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, United States of America.
Holly R MiddlekauffDepartment of Physiology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, United States of America; Division of Cardiology, Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, United States of America.
University of California, Los Angeles · US

Funding

UCLA Clinical and Translational Science InstituteUL1TR000124 · NCATS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI DUBINETT, STEVEN M. · 2012 to 2015
$57.0M
MODULATION OF SARCOPLASMIC RETICULUM CALCIUM RELEASER01AR041802 · NIAMS · BAYLOR COLLEGE OF MEDICINE · PI HAMILTON, SUSAN L, INGALLS, CHRISTOPHER · 1993 to 2014
$5.6M
Excitation-Contraction Coupling in Normal and Dystrophic Mammalian MuscleR01AR047664 · NIAMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI VERGARA, JULIO L · 2003 to 2013
$2.7M
Role of the Transverse Tubular System in Mammalian Skeletal Muscle ExcitabilityR01AR054816 · NIAMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI VERGARA, JULIO L · 2007 to 2011
$1.6M
Excitation-contraction Coupling in Normal and Dystrophic Mammalian MuscleR56AR047664 · NIAMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI VERGARA, JULIO L · 2008 to 2008
$339k
NCATS NIH HHS UL1 TR000124NCATS NIH HHS UL1TR000124NIAMS NIH HHS AR041802NIAMS NIH HHS AR047664NIAMS NIH HHS AR54816NIAMS NIH HHS R01 AR041802NIAMS NIH HHS R01 AR047664NIAMS NIH HHS R01 AR054816NIAMS NIH HHS R56 AR047664
6 · The paper itself

Abstract

backgroundExercise intolerance in chronic heart failure (HF) has been attributed to abnormalities of the skeletal muscles. Muscle function depends on intact excitation-contraction coupling (ECC), but ECC studies in HF models have been inconclusive, due to deficiencies in the animal models and tools used to measure calcium (Ca2+) release, mandating investigations in skeletal muscle from HF patients. The purpose of this study was to test the hypothesis that Ca2+ release is significantly impaired in the skeletal muscle of HF patients in whom exercise capacity is severely diminished compared to age-matched healthy volunteers. METHODS AND

findingsUsing state-of-the-art electrophysiological and optical techniques in single muscle fibers from biopsies of the locomotive vastus lateralis muscle, we measured the action potential (AP)-evoked Ca2+ release in 4 HF patients and 4 age-matched healthy controls. The mean peak Ca2+ release flux in fibers obtained from HF patients (10±1.2 µM/ms) was markedly (2.6-fold) and significantly (p<0.05) smaller than in fibers from healthy volunteers (28±3.3 µM/ms). This impairment in AP-evoked Ca2+ release was ubiquitous and was not explained by differences in the excitability mechanisms since single APs were indistinguishable between HF patients and healthy volunteers.

conclusionsThese findings prove the feasibility of performing electrophysiological experiments in single fibers from human skeletal muscle, and offer a new approach for investigations of myopathies due to HF and other diseases. Importantly, we have demonstrated that one step in the ECC process, AP-evoked Ca2+ release, is impaired in single muscle fibers in HF patients.

Indexed as

Action PotentialsCalciumFemaleHeart FailureHumansMaleMiddle AgedMuscle Fibers, SkeletalMuscle, SkeletalCalcium

Identifiers

PMID25310188
PMCPMC4195605
OpenAlexW1972683461

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.