ArticleFrontiers in oncology2014
Laser microdissection of the alveolar duct enables single-cell genomic analysis.
Article in Frontiers in oncology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 9 citations in OpenAlex.
- Chemotherapy-Mediated Purging Before Testicular Organoid Generation Significantly Reduces Cancer Cells While Not Significantly Reducing Germ Cells.International journal of molecular sciences · 2026Article
- Intracellular Amyloid-β Detection from Human Brain Sections Using a Microfluidic Immunoassay in Tandem with MALDI-MS.Analytical chemistry · 2023Article
- Single-Cell Transcriptional Profiling of Cells Derived From Regenerating Alveolar Ducts.Frontiers in medicine · 2020Article
- In vitro and ex vivo systems at the forefront of infection modeling and drug discovery.Biomaterials · 2019Review
- Deformation-induced transitional myofibroblasts contribute to compensatory lung growth.American journal of physiology. Lung cellular and molecular physiology · 2017Article
- Microfluidic Sample Preparation for Single Cell Analysis.Analytical chemistry · 2016Review
- Elastin Cables Define the Axial Connective Tissue System in the Murine Lung.Anatomical record (Hoboken, N.J. : 2007) · 2015Article
- Impact of recent innovations in the use of mass cytometry in support of drug development.Drug discovery today · 2015Article
- Remodeling of alveolar septa after murine pneumonectomy.American journal of physiology. Lung cellular and molecular physiology · 2015Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
Abstract
Complex tissues such as the lung are composed of structural hierarchies such as alveoli, alveolar ducts, and lobules. Some structural units, such as the alveolar duct, appear to participate in tissue repair as well as the development of bronchioalveolar carcinoma. Here, we demonstrate an approach to conduct laser microdissection of the lung alveolar duct for single-cell PCR analysis. Our approach involved three steps. (1) The initial preparation used mechanical sectioning of the lung tissue with sufficient thickness to encompass the structure of interest. In the case of the alveolar duct, the precision-cut lung slices were 200 μm thick; the slices were processed using near-physiologic conditions to preserve the state of viable cells. (2) The lung slices were examined by transmission light microscopy to target the alveolar duct. The air-filled lung was sufficiently accessible by light microscopy that counterstains or fluorescent labels were unnecessary to identify the alveolar duct. (3) The enzymatic and microfluidic isolation of single cells allowed for the harvest of as few as several thousand cells for PCR analysis. Microfluidics based arrays were used to measure the expression of selected marker genes in individual cells to characterize different cell populations. Preliminary work suggests the unique value of this approach to understand the intra- and intercellular interactions within the regenerating alveolar duct.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.