ArticleApplications in plant sciences2014
Paramagnetic cellulose DNA isolation improves DNA yield and quality among diverse plant taxa.
Article in Applications in plant sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Comparing DNA isolation methods for forest trees: quality, plastic footprint, and time-efficiency.Plant methods · 2023Article
- An efficient method for high molecular weight bacterial DNA extraction suitable for shotgun metagenomics from skin swabs.Microbial genomics · 2023Article
- Integration of sample preparation with RNA-Amplification in a hand-held device for airborne virus detection.Analytica chimica acta · 2021Article
- Evaluation and application of an efficient plant DNA extraction protocol for laboratory and field testing.Journal of Zhejiang University. Science. B · 2021Article
- Rapid (30-second), equipment-free purification of nucleic acids using easy-to-make dipsticks.Nature protocols · 2020Article
- Impact of DNA Extraction Method on Variation in Human and Built Environment Microbial Community and Functional Profiles Assessed by Shotgun Metagenomics Sequencing.Frontiers in microbiology · 2020Article
- Enhanced solid phase extraction of DNA using hydrophilic monodisperse poly(methacrylic acid-co-ethylene dimethacrylate) microparticles.Molecular biology reports · 2019Article
- Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes.Journal of visualized experiments : JoVE · 2018Article
- Automated DNA extraction using cellulose magnetic beads can improveOncotarget · 2018Article
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Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
unlabelledPREMISE OF THE STUDY: The chemical diversity of land plants ensures that no single DNA isolation method results in high yield and purity with little effort for all species. Here we evaluate a new technique originally developed for forensic science, based on MagnaCel paramagnetic cellulose particles (PMC), to determine its efficacy in extracting DNA from 25 plant species representing 21 families and 15 orders. • METHODS AND
resultsYield and purity of DNA isolated by PMC, DNeasy Plant Mini Kit (silica column), and cetyltrimethylammonium bromide (CTAB) methods were compared among four individuals for each of 25 plant species. PMC gave a twofold advantage in average yield, and the relative advantage of the PMC method was greatest for samples with the lowest DNA yields. PMC also produced more consistent sample purity based on absorbance ratios at 260:280 and 260:230 nm. •
conclusionsPMC technology is a promising alternative for plant DNA isolation.
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