ArticlePlant methods2014
Using monoclonal antibodies to label living root hairs: a novel tool for studying cell wall microarchitecture and dynamics in Arabidopsis.
Article in Plant methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 24 citations in OpenAlex.
- The Localization of Cell Wall Components in the Whole-Mount ImmunolabeledInternational journal of molecular sciences · 2025Article
- Cell wall-related glycosyltransferases and wall architecture in the model liverwort Marchantia polymorpha.The Plant journal : for cell and molecular biology · 2025Article
- The structure and interaction of polymers affects secondary cell wall banding patterns in Arabidopsis.The Plant cell · 2024Article
- Do Cuticular Gaps Make It Possible to Study the Composition of the Cell Walls in the Glands ofInternational journal of molecular sciences · 2024Article
- Growth-inhibiting effects of the unconventional plant APYRASE 7 of Arabidopsis thaliana influences the LRX/RALF/FER growth regulatory module.PLoS genetics · 2024Article
- The Localization of Cell Wall Components in the Quadrifids of Whole-Mount ImmunolabeledInternational journal of molecular sciences · 2023Article
- Polyclonal Aptamer Libraries from a FluRoot-SELEX for the Specific Labeling of the Apical and Elongation/Differentiation Zones ofInternational journal of molecular sciences · 2022Article
- Development of plant systemic resistance by beneficial rhizobacteria: Recognition, initiation, elicitation and regulation.Frontiers in plant science · 2022Review
- High-resolution 3D mapping of rhizosphere glycan patterning using molecular probes in a transparent soil system.Cell surface (Amsterdam, Netherlands) · 2021Article
- Root hairs enhance Arabidopsis seedling survival upon soil disruption.Scientific reports · 2019Article
- Evolution of Cell Wall Polymers in Tip-Growing Land Plant Gametophytes: Composition, Distribution, Functional Aspects and Their Remodeling.Frontiers in plant science · 2019Review
- Report on the Current Inventory of the Toolbox for Plant Cell Wall Analysis: Proteinaceous and Small Molecular Probes.Frontiers in plant science · 2018Review
- The Root Hair Specific SYP123 Regulates the Localization of Cell Wall Components and Contributes to Rizhobacterial Priming of Induced Systemic Resistance.Frontiers in plant science · 2016Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe Arabidopsis root hair represents a valuable cell model for elucidating polar expansion mechanisms in plant cells and the overall biology of roots. The deposition and development of the cell wall is central to the root hair expansion apparatus. During this process, incorporation of specific wall polymers into the growing wall architecture constitutes a critical spatio-temporal event that controls hair size and growth rate and one that is closely coordinated with the cell's endomembrane, cytoskeletal and signal transduction apparatuses.
resultsIn this study, the protocol for live cell labeling of roots with monoclonal antibodies that bind to specific wall polymers is presented. This method allows for rapid assessment of root hair cell wall composition during development and assists in describing changes to cell wall composition in transgenic mutant lines. Enzymatic "unmasking" of specific polymers prior to labeling allows for refined interpretation of cell wall chemistry. Live cell immunofluorescence data may also be correlated with transmission electron microscopy-based immunogold labeling.
conclusionsLive Arabidopsis root hairs may be labeled with cell wall polymer-specific antibodies. This methodology allows for direct visualization of cell wall dynamics throughout development in stable transgenic plant lines. It also provides an important new tool in the elucidation of the specific interactions occurring between membrane trafficking networks, cytoskeleton and the cell wall deposition/remodeling mechanism.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.