ArticleIEEE journal of selected topics in quantum electronics : a publication of the IEEE Lasers and Electro-optics Society2014
Silicon photon-counting avalanche diodes for single-molecule fluorescence spectroscopy.
Article in IEEE journal of selected topics in quantum electronics : a publication of the IEEE Lasers and Electro-optics Society, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 63 citations in OpenAlex.
- Fast Gating for Raman Spectroscopy.Sensors (Basel, Switzerland) · 2021Review
- High-throughput smFRET analysis of freely diffusing nucleic acid molecules and associated proteins.Methods (San Diego, Calif.) · 2019Article
- Single-photon avalanche diode imagers in biophotonics: review and outlook.Light, science & applications · 2019Review
- Optical crosstalk in SPAD arrays for high-throughput single-molecule fluorescence spectroscopy.Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment · 2018Article
- 48-spot single-molecule FRET setup with periodic acceptor excitation.The Journal of chemical physics · 2018Article
- Red-Enhanced Photon Detection Module Featuring a 32 × 1 Single-Photon Avalanche Diode Array.IEEE photonics technology letters : a publication of the IEEE Laser and Electro-optics Society · 2018Article
- Toward dynamic structural biology: Two decades of single-molecule Förster resonance energy transfer.Science (New York, N.Y.) · 2018Review
- Infrared single photon detector based on optical up-converter at 1550 nm.Scientific reports · 2017Article
- Development and characterization of an 8×8 SPAD-array module for gigacount per second applications.Proceedings of SPIE--the International Society for Optical Engineering · 2017Article
- 16-Ch Time-resolved Single-Molecule Spectroscopy Using Line Excitation.Proceedings of SPIE--the International Society for Optical Engineering · 2017Article
- Multispot single-molecule FRET: High-throughput analysis of freely diffusing molecules.PloS one · 2017Article
- High-voltage integrated active quenching circuit for single photon count rate up to 80 Mcounts/s.Optics express · 2016Article
- Gigacount/second Photon Detection Module Based on an 8 × 8 Single-Photon Avalanche Diode Array.IEEE photonics technology letters : a publication of the IEEE Laser and Electro-optics Society · 2016Article
- Silicon technologies for arrays of Single Photon Avalanche Diodes.Proceedings of SPIE--the International Society for Optical Engineering · 2016Article
- Photon-HDF5: An Open File Format for Timestamp-Based Single-Molecule Fluorescence Experiments.Biophysical journal · 2016Article
- Improving the counting efficiency in time-correlated single photon counting experiments by dead-time optimization.The Review of scientific instruments · 2015Review
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Solution-based single-molecule fluorescence spectroscopy is a powerful experimental tool with applications in cell biology, biochemistry and biophysics. The basic feature of this technique is to excite and collect light from a very small volume and work in a low concentration regime resulting in rare burst-like events corresponding to the transit of a single molecule. Detecting photon bursts is a challenging task: the small number of emitted photons in each burst calls for high detector sensitivity. Bursts are very brief, requiring detectors with fast response time and capable of sustaining high count rates. Finally, many bursts need to be accumulated to achieve proper statistical accuracy, resulting in long measurement time unless parallelization strategies are implemented to speed up data acquisition. In this paper we will show that silicon single-photon avalanche diodes (SPADs) best meet the needs of single-molecule detection. We will review the key SPAD parameters and highlight the issues to be addressed in their design, fabrication and operation. After surveying the state-of-the-art SPAD technologies, we will describe our recent progress towards increasing the throughput of single-molecule fluorescence spectroscopy in solution using parallel arrays of SPADs. The potential of this approach is illustrated with single-molecule Förster resonance energy transfer measurements.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.