ArticleActa biomaterialia2015
Matrix RGD ligand density and L1CAM-mediated Schwann cell interactions synergistically enhance neurite outgrowth.
Article in Acta biomaterialia, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 47 citations in OpenAlex.
- Directional guidance to orient Schwann cell alignment in nerve regeneration requires Plexin-B1.Science advances · 2026Article
- Viscoelastic N‑cadherin-like interactions maintain neural progenitor cell stemness within 3D matrices.Nature communications · 2025Article
- NJournal of experimental & clinical cancer research : CR · 2025Article
- Recent advances in enhances peripheral nerve orientation: the synergy of micro or nano patterns with therapeutic tactics.Journal of nanobiotechnology · 2024Review
- 3D bioprinting of dynamic hydrogel bioinks enabled by small molecule modulators.Science advances · 2023Article
- Synthetic Extracellular Matrices for 3D Culture of Schwann Cells, Hepatocytes, and HUVECs.Bioengineering (Basel, Switzerland) · 2022Article
- Article
- Elastin-like Proteins to Support Peripheral Nerve Regeneration in Guidance Conduits.ACS biomaterials science & engineering · 2021Article
- Schwann cell interactions during the development of the peripheral nervous system.Developmental neurobiology · 2021Review
- Spidroin Silk Fibers with Bioactive Motifs of Extracellular Proteins for Neural Tissue Engineering.ACS omega · 2021Article
- Epitranscriptomic Analysis of m6A Methylome After Peripheral Nerve Injury.Frontiers in genetics · 2021Article
- Neural Progenitor Cells Alter Chromatin Organization and Neurotrophin Expression in Response to 3D Matrix Degradability.Advanced healthcare materials · 2020Article
- Designer, injectable gels to prevent transplanted Schwann cell loss during spinal cord injury therapy.Science advances · 2020Article
- Biomimetic Approaches for Separated Regeneration of Sensory and Motor Fibers in Amputee People: Necessary Conditions for Functional Integration of Sensory-Motor Prostheses With the Peripheral Nerves.Frontiers in bioengineering and biotechnology · 2020Article
- Matrix Remodeling Enhances the Differentiation Capacity of Neural Progenitor Cells in 3D Hydrogels.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2019Article
- Tissue Models for Neurogenesis and Repair in 3D.Advanced functional materials · 2018Article
- Photocrosslinkable Gelatin/Tropoelastin Hydrogel Adhesives for Peripheral Nerve Repair.Tissue engineering. Part A · 2018Article
- Investigating the interplay between substrate stiffness and ligand chemistry in directing mesenchymal stem cell differentiation within 3D macro-porous substrates.Biomaterials · 2018Article
- Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D.Journal of visualized experiments : JoVE · 2018Article
- Maintenance of neural progenitor cell stemness in 3D hydrogels requires matrix remodelling.Nature materials · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The innate biological response to peripheral nerve injury involves a complex interplay of multiple molecular cues to guide neurites across the injury gap. Many current strategies to stimulate regeneration take inspiration from this biological response. However, little is known about the balance of cell-matrix and Schwann cell-neurite dynamics required for regeneration of neural architectures. We present an engineered extracellular matrix (eECM) microenvironment with tailored cell-matrix and cell-cell interactions to study their individual and combined effects on neurite outgrowth. This eECM regulates cell-matrix interactions by presenting integrin-binding RGD (Arg-Gly-Asp) ligands at specified densities. Simultaneously, the addition or exclusion of nerve growth factor (NGF) is used to modulate L1CAM-mediated Schwann cell-neurite interactions. Individually, increasing the RGD ligand density from 0.16 to 3.2mM resulted in increasing neurite lengths. In matrices presenting higher RGD ligand densities, neurite outgrowth was synergistically enhanced in the presence of soluble NGF. Analysis of Schwann cell migration and co-localization with neurites revealed that NGF enhanced cooperative outgrowth between the two cell types. Interestingly, neurites in NGF-supplemented conditions were unable to extend on the surrounding eECM without the assistance of Schwann cells. Blocking studies revealed that L1CAM is primarily responsible for these Schwann cell-neurite interactions. Without NGF supplementation, neurite outgrowth was unaffected by L1CAM blocking or the depletion of Schwann cells. These results underscore the synergistic interplay between cell-matrix and cell-cell interactions in enhancing neurite outgrowth for peripheral nerve regeneration.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.