ArticleYeast (Chichester, England)2014
The yeast enzyme Eht1 is an octanoyl-CoA:ethanol acyltransferase that also functions as a thioesterase.
Article in Yeast (Chichester, England), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 42 citations in OpenAlex.
- Production and Characterization of Kefir Beverages by Fermentation of Whole Milk with Milk or Water Kefir Grains.Foods (Basel, Switzerland) · 2026Article
- Genome-based exploration of volatile flavor diversity from food yeast species.FEMS yeast research · 2025Review
- Towards biotechnological production of bio-based low molecular weight esters: a patent review.RSC advances · 2024Review
- Screening low-methanol and high-aroma produced yeasts for cider fermentation by transcriptive characterization.Frontiers in microbiology · 2022Article
- Selection by UV Mutagenesis and Physiological Characterization of Mutant Strains of the YeastJournal of fungi (Basel, Switzerland) · 2021Article
- Metabolic, Organoleptic and Transcriptomic Impact ofInternational journal of molecular sciences · 2021Article
- The Role of Yeasts and Lactic Acid Bacteria on the Metabolism of Organic Acids during Winemaking.Foods (Basel, Switzerland) · 2020Review
- Article
- Contribution of Eat1 and Other Alcohol Acyltransferases to Ester Production inFrontiers in microbiology · 2018Article
- Saccharomyces cerevisiae Atf1p is an alcohol acetyltransferase and a thioesterase in vitro.Yeast (Chichester, England) · 2017Article
- Engineering fungal de novo fatty acid synthesis for short chain fatty acid production.Nature communications · 2017Article
- Monoacylglycerol Lipases Act as Evolutionarily Conserved Regulators of Non-oxidative Ethanol Metabolism.The Journal of biological chemistry · 2016Article
- Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Fatty acid ethyl esters are secondary metabolites that are produced during microbial fermentation, in fruiting plants and in higher organisms during ethanol stress. In particular, volatile medium-chain fatty acid ethyl esters are important flavour compounds that impart desirable fruit aromas to fermented beverages, including beer and wine. The biochemical synthesis of medium-chain fatty acid ethyl esters is poorly understood but likely involves acyl-CoA:ethanol O-acyltransferases. Here, we characterize the enzyme ethanol hexanoyl transferase 1 (Eht1) from the brewer's yeast Saccharomyces cerevisiae. Full-length Eht1 was successfully overexpressed from a recombinant yeast plasmid and purified at the milligram scale after detergent solubilization of sedimenting membranes. Recombinant Eht1 was functional as an acyltransferase and, unexpectedly, was optimally active toward octanoyl-CoA, with k(cat) = 0.28 ± 0.02/s and K(M) = 1.9 ± 0.6 μm. Eht1 was also revealed to be active as a thioesterase but was not able to hydrolyse p-nitrophenyl acyl esters, in contrast to the findings of a previous study. Low-resolution structural data and site-directed mutagenesis provide experimental support for a predicted α/β-hydrolase domain featuring a Ser-Asp-His catalytic triad. The S. cerevisiae gene YBR177C/EHT1 should thus be reannotated as coding for an octanoyl-CoA:ethanol acyltransferase that can also function as a thioesterase.
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