ArticleThe Journal of molecular diagnostics : JMD2014
Seven novel probe systems for real-time PCR provide absolute single-base discrimination, higher signaling, and generic components.
Article in The Journal of molecular diagnostics : JMD, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 15 citations in OpenAlex.
- Advances in Diagnostic Techniques for Influenza Virus Infection: A Comprehensive Review.Tropical medicine and infectious disease · 2025Review
- A Multiplex PCR Melting-Curve-Analysis-Based Detection Method for the Discrimination of FiveJournal of fungi (Basel, Switzerland) · 2023Article
- Genomic alterations in cholangiocarcinoma: clinical significance and relevance to therapy.Exploration of targeted anti-tumor therapy · 2022Review
- Nucleic Acid-Based Sensing Techniques for Diagnostics and Surveillance of Influenza.Biosensors · 2021Review
- Phosphodiesterase-induced cAMP degradation restricts hepatitis B virus infection.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- Functional evaluation of a PTSD-associated genetic variant: estradiol regulation and ADCYAP1R1.Translational psychiatry · 2016Article
- Multiplex qPCR for serodetection and serotyping of hepatitis viruses: A brief review.World journal of gastroenterology · 2016Review
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3 authors at 2 institutions in 1 country.
Funding
Abstract
We have developed novel probe systems for real-time PCR that provide higher specificity, greater sensitivity, and lower cost relative to dual-labeled probes. The seven DNA Detection Switch (DDS)-probe systems reported here employ two interacting polynucleotide components: a fluorescently labeled probe and a quencher antiprobe. High-fidelity detection is achieved with three DDS designs: two internal probes (internal DDS and Flip probes) and a primer probe (ZIPR probe), wherein each probe is combined with a carefully engineered, slightly mismatched, error-checking antiprobe. The antiprobe blocks off-target detection over a wide range of temperatures and facilitates multiplexing. Other designs (Universal probe, Half-Universal probe, and MacMan probe) use generic components that enable low-cost detection. Finally, single-molecule G-Force probes employ guanine-mediated fluorescent quenching by forming a hairpin between adjacent C-rich and G-rich sequences. Examples provided show how these probe technologies discriminate drug-resistant Mycobacterium tuberculosis mutants, Escherichia coli O157:H7, oncogenic EGFR deletion mutations, hepatitis B virus, influenza A/B strains, and single-nucleotide polymorphisms in the human VKORC1 gene.
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