ArticleBMC genomics2014
Impact of whole-genome amplification on the reliability of pre-transfer cattle embryo breeding value estimates.
Article in BMC genomics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 34 citations in OpenAlex.
- Establishment and validation of porcine embryo genomic selection system through SCNT technique.Journal of animal science and biotechnology · 2026Article
- Genomic evaluation and gene expression analysis of preimplantation embryos for enhancing cattle breeding and in vitro embryo production.The Journal of reproduction and development · 2026Review
- Handmade Biopsy for Genotyping of In Vitro-Produced Bovine Embryos.Anatomia, histologia, embryologia · 2026Article
- Article
- Toward Practical Embryo Genomic Selection in Cattle: An Optimized Workflow Enabling High-Accuracy Breeding Value Prediction Without Compromising Embryo Survival.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
- The Establishment of a Sheep Embryo Genomic Selection System.International journal of molecular sciences · 2025Article
- From the laboratory to the field: how to mitigate pregnancy losses in embryo transfer programs?Animal reproduction · 2024Review
- Pre-Implantation Bovine Embryo Evaluation-From Optics to Omics and Beyond.Animals : an open access journal from MDPI · 2023Review
- Embryo biopsies for genomic selection in tropical dairy cattle.Animal reproduction · 2023Review
- Enhancing Genetic Gain through Genomic Selection: From Livestock to Plants.Plant communications · 2020Review
- Potential of preimplantation genomic selection for carcass traits in Japanese Black cattle.The Journal of reproduction and development · 2019Article
- Accelerating crop genetic gains with genomic selection.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Review
- From clinics to (cow)mics: a reproductive journey.Animal reproduction · 2018Article
- Production of calves by the transfer of cryopreserved bovine elongating conceptuses and possible application for preimplantation genomic selection.The Journal of reproduction and development · 2017Article
- Improved DOP-PCR (iDOP-PCR): A robust and simple WGA method for efficient amplification of low copy number genomic DNA.PloS one · 2017Article
- Holsteins are the genomic selection poster cows.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGenome-wide profiling of single-nucleotide polymorphisms is receiving increasing attention as a method of pre-implantation genetic diagnosis in humans and of commercial genotyping of pre-transfer embryos in cattle. However, the very small quantity of genomic DNA in biopsy material from early embryos poses daunting technical challenges. A reliable whole-genome amplification (WGA) procedure would greatly facilitate the procedure.
resultsSeveral PCR-based and non-PCR based WGA technologies, namely multiple displacement amplification, quasi-random primed library synthesis followed by PCR, ligation-mediated PCR, and single-primer isothermal amplification were tested in combination with different DNA extractions protocols for various quantities of genomic DNA inputs. The efficiency of each method was evaluated by comparing the genotypes obtained from 15 cultured cells (representative of an embryonic biopsy) to unamplified reference gDNA. The gDNA input, gDNA extraction method and amplification technology were all found to be critical for successful genome-wide genotyping. The selected WGA platform was then tested on embryo biopsies (n = 226), comparing their results to that of biopsies collected after birth. Although WGA inevitably leads to a random loss of information and to the introduction of erroneous genotypes, following genomic imputation the resulting genetic index of both sources of DNA were highly correlated (r = 0.99, P<0.001).
conclusionIt is possible to generate high-quality DNA in sufficient quantities for successful genome-wide genotyping starting from an early embryo biopsy. However, imputation from parental and population genotypes is a requirement for completing and correcting genotypic data. Judicious selection of the WGA platform, careful handling of the samples and genomic imputation together, make it possible to perform extremely reliable genomic evaluations for pre-transfer embryos.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.