ArticleMicrobiologyOpen2014
Genetic and phenotypic characterization of the heat shock response in Pseudomonas putida.
Article in MicrobiologyOpen, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 25 citations in OpenAlex.
- SIGHT-A System for Solvent-Tight Incubation and Growth Monitoring in High Throughput.Engineering in life sciences · 2025Article
- Synthesis and Degradation of the Phytohormone Indole-3-Acetic Acid by the Versatile BacteriumPlants (Basel, Switzerland) · 2024Article
- Cytoplasmic molecular chaperones in Pseudomonas species.Journal of microbiology (Seoul, Korea) · 2022Review
- Cell Envelope Stress Response in Pseudomonas aeruginosa.Advances in experimental medicine and biology · 2022Article
- Elucidating Essential Genes in Plant-Associated Pseudomonas protegens Pf-5 Using Transposon Insertion Sequencing.Journal of bacteriology · 2021Article
- The Role of ClpB in Bacterial Stress Responses and Virulence.Frontiers in molecular biosciences · 2021Review
- LeveragingFrontiers in microbiology · 2021Review
- Transcriptomic determinants of the response of ST-111 Pseudomonas aeruginosa AG1 to ciprofloxacin identified by a top-down systems biology approach.Scientific reports · 2020Article
- Synthetic control of plasmid replication enables target- and self-curing of vectors and expedites genome engineering ofMetabolic engineering communications · 2020Article
- Construction and characterization of broad-host-range reporter plasmid suitable for on-line analysis of bacterial host responses related to recombinant protein production.Microbial cell factories · 2019Article
- Novel non-phosphorylative pathway of pentose metabolism from bacteria.Scientific reports · 2019Article
- Comparative roles of clpA and clpB in the survival of S. Typhimurium under stress and virulence in poultry.Scientific reports · 2018Article
- Characterization of a NovelJournal of bacteriology · 2017Article
- Functional characterization of aconitase X as a cis-3-hydroxy-L-proline dehydratase.Scientific reports · 2016Article
- Transcriptome analysis of Pseudomonas aeruginosa PAO1 grown at both body and elevated temperatures.PeerJ · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Molecular chaperones function in various important physiological processes. Null mutants of genes for the molecular chaperone ClpB (Hsp104), and those that encode J-domain proteins (DnaJ, CbpA, and DjlA), which may act as Hsp40 co-chaperones of DnaK (Hsp70), were constructed from Pseudomonas putida KT2442 (KT) to elucidate their roles. The KTΔclpB mutant showed the same heat shock response (HSR) as the wild-type, both in terms of heat-shock protein (Hsp) synthesis (other than ClpB) and in hsp gene expression; however, the mutant was quite sensitive to high temperatures and was unable to disaggregate into thermo-mediated protein aggregates, indicating that ClpB is important for cell survival after heat stress and essential for solubilization of protein aggregates. On the other hand, the KTΔdnaJ mutant was temperature-sensitive, and formed more protein aggregates (especially of high molecular weight) upon heat stress than did KT. P. putida CbpA, a probable Hsp, partially substituted the functions of DnaJ in cell growth and solubilization of thermo-mediated protein aggregates, and might be involved in the HSR which was regulated by a fine-tuning system(s) that could sense subtle changes in the ambient temperature and control the levels of σ(32) activity and quantity, as well as the mRNA levels of hsp genes.
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