ArticlePloS one2014
The AS87_04050 gene is involved in bacterial lipopolysaccharide biosynthesis and pathogenicity of Riemerella anatipestifer.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 37 citations in OpenAlex.
- Characterization of Riemerella anatipestifer in broiler chickens from Shandong, China: serotype, antimicrobial resistance, and virulence gene profiles.Poultry science · 2026Article
- Characterization of the Chicken-Derived Riemerella anatipestifer Strains in Beijing and Fujian Provinces, China.Transboundary and emerging diseases · 2026Article
- Research Progress in the Development of Vaccines AgainstMicroorganisms · 2025Review
- Isolation, identification, and proteomic analysis of outer membrane vesicles of Riemerella anatipestifer SX-1.Poultry science · 2024Article
- Occludin and collagen IV degradation mediated by the T9SS effector SspA contributes to blood-brain barrier damage in ducks during Riemerella anatipestifer infection.Veterinary research · 2024Article
- Genome-wide association study reveals serovar-associated genetic loci in Riemerella anatipestifer.BMC genomics · 2024Article
- A Riemerella anatipestifer Metallophosphoesterase That Displays Phosphatase Activity and Is Associated with Virulence.Applied and environmental microbiology · 2021Article
- Anti-inflammatory activity of diindolylmethane alleviates Riemerella anatipestifer infection in ducks.PloS one · 2020Article
- Lsr operon is associated with AI-2 transfer and pathogenicity in avian pathogenic Escherichia coli.Veterinary research · 2019Article
- New Perspectives on Galleria mellonella Larvae as a Host Model Using Riemerella anatipestifer as a Proof of Concept.Infection and immunity · 2019Article
- Riemerella anatipestifer GldM is required for bacterial gliding motility, protein secretion, and virulence.Veterinary research · 2019Article
- Article
- Riemerella anatipestifer AS87_RS09170 gene is responsible for biotin synthesis, bacterial morphology and virulence.Scientific reports · 2018Article
- The Riemerella anatipestifer M949_RS01035 gene is involved in bacterial lipopolysaccharide biosynthesis.Veterinary research · 2018Article
- Disruption of the M949_RS01915 gene changed the bacterial lipopolysaccharide pattern, pathogenicity and gene expression of Riemerella anatipestifer.Veterinary research · 2017Article
- The Riemerella anatipestifer AS87_01735 Gene Encodes Nicotinamidase PncA, an Important Virulence Factor.Applied and environmental microbiology · 2016Article
- Deletion of AS87_03730 gene changed the bacterial virulence and gene expression of Riemerella anatipestifer.Scientific reports · 2016Article
- Identification and Characterization of anFrontiers in microbiology · 2016Article
- Riemerella anatipestifer M949_1360 Gene Functions on the Lipopolysaccharide Biosynthesis and Bacterial Virulence.PloS one · 2016Article
- Genome Sequence of Avirulent Riemerella anatipestifer Strain RA-JLLY.Genome announcements · 2015Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Riemerella anatipestifer is reported worldwide as a cause of septicemic and exudative diseases of domestic ducks. In this study, we identified a mutant strain RA2640 by Tn4351 transposon mutagenesis, in which the AS87_04050 gene was inactivated by insertion of the transposon. Southern blot analysis indicated that only one insertion was found in the genome of the mutant strain RA2640. SDS-PAGE followed by silver staining showed that the lipopolysaccharide (LPS) pattern of mutant strain RA2640 was different from its wild-type strain Yb2, suggesting the LPS was defected. In addition, the phenotype of the mutant strain RA2640 was changed to rough-type, evident by altered colony morphology, autoaggregation ability and crystal violet staining characteristics. Bacterial LPS is a key factor in virulence as well as in both innate and acquired host responses to infection. The rough-type mutant strain RA2640 showed higher sensitivity to antibiotics, disinfectants and normal duck serum, and higher capability of adherence and invasion to Vero cells, compared to its wild-type strain Yb2. Moreover, the mutant strain RA2640 lost the agglutination ability of its wild-type strain Yb2 to R. anatipestifer serotype 2 positive sera, suggesting that the O-antigen is defected. Animal experiments indicated that the virulence of the mutant strain RA2640 was attenuated by more than 100,000-fold, compared to its wild-type strain Yb2. These results suggested that the AS87_04050 gene in R. anatipestifer is associated with the LPS biosynthesis and bacterial pathogenicity.
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