ArticlePlant physiology2014
Dual catalytic activity of hydroxycinnamoyl-coenzyme A quinate transferase from tomato allows it to moonlight in the synthesis of both mono- and dicaffeoylquinic acids.
Article in Plant physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 69 citations in OpenAlex.
- Completing the acylation landscape in plants: GDSL enzymes join the BAHD and SCPL acyltransferase families.Journal of experimental botany · 2026Review
- Advances in the Function Roles of Hydroxycinnamoyl-CoA Shikimate/Quinate Hydroxycinnamoyl Transferases: A Key Enzyme Linking Phenylpropanoid Metabolism to Plant Terrestrial Adaptation.Plants (Basel, Switzerland) · 2026Review
- Genome-wide analysis of BAHD gene family and in vivo characterization of HQT-like genes involved in chlorogenic acid synthesis in tobacco (Nicotiana tabacum L.).BMC plant biology · 2026Article
- Recent Advances in Biosynthesis and Bioactivity of Plant Caffeoylquinic Acids.Current issues in molecular biology · 2025Review
- Molecular Identification and Functional Characterization of a Putrescine Hydroxycinnamoyltransferase in Suspension Cells of Bamboo (Phyllostachys nigra).Applied biochemistry and biotechnology · 2025Article
- Integrated metabolomic and transcriptomic analysis of the anthocyanin regulatory networks in Lagerstroemia indica petals.BMC plant biology · 2025Article
- Phenolic metabolism in Sarcandra glabra is mediated by distinct BAHD hydroxycinnamoyltransferases.The Plant journal : for cell and molecular biology · 2025Article
- Identification of the first plant caffeoyl-quinate esterases inFrontiers in plant science · 2025Article
- The effects of clonal integration and earthworms on the growth, active constituent accumulation inFrontiers in plant science · 2025Article
- Article
- Risk Assessment of Chlorogenic and Isochlorogenic Acids in Coffee By-Products.Molecules (Basel, Switzerland) · 2023Review
- Genetics and breeding of phenolic content in tomato, eggplant and pepper fruits.Frontiers in plant science · 2023Review
- Exploring the metabolic and physiological roles ofFrontiers in plant science · 2023Article
- Family characteristics, phylogenetic reconstruction, and potential applications of the plant BAHD acyltransferase family.Frontiers in plant science · 2023Review
- Advances in Production of Hydroxycinnamoyl-Quinic Acids: From Natural Sources to Biotechnology.Antioxidants (Basel, Switzerland) · 2022Review
- Callus Culture ofMolecules (Basel, Switzerland) · 2022Article
- Comparative transcriptome and weighted correlation network analyses reveal candidate genes involved in chlorogenic acid biosynthesis in sweet potato.Scientific reports · 2022Article
- The Evolution of Leaf Function during Development Is Reflected in Profound Changes in the Metabolic Composition of the Vacuole.Metabolites · 2021Article
- Caffeoylquinic acids: chemistry, biosynthesis, occurrence, analytical challenges, and bioactivity.The Plant journal : for cell and molecular biology · 2021Review
- Versatility in acyltransferase activity completes chicoric acid biosynthesis in purple coneflower.Nature communications · 2021Article
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Tomato (Solanum lycopersicum), like other Solanaceous species, accumulates high levels of antioxidant caffeoylquinic acids, which are strong bioactive molecules and protect plants against biotic and abiotic stresses. Among these compounds, the monocaffeoylquinic acids (e.g. chlorogenic acid [CGA]) and the dicaffeoylquinic acids (diCQAs) have been found to possess marked antioxidative properties. Thus, they are of therapeutic interest both as phytonutrients in foods and as pharmaceuticals. Strategies to increase diCQA content in plants have been hampered by the modest understanding of their biosynthesis and whether the same pathway exists in different plant species. Incubation of CGA with crude extracts of tomato fruits led to the formation of two new products, which were identified by liquid chromatography-mass spectrometry as diCQAs. This chlorogenate:chlorogenate transferase activity was partially purified from ripe fruit. The final protein fraction resulted in 388-fold enrichment of activity and was subjected to trypsin digestion and mass spectrometric sequencing: a hydroxycinnamoyl-Coenzyme A:quinate hydroxycinnamoyl transferase (HQT) was selected as a candidate protein. Assay of recombinant HQT protein expressed in Escherichia coli confirmed its ability to synthesize diCQAs in vitro. This second activity (chlorogenate:chlorogenate transferase) of HQT had a low pH optimum and a high Km for its substrate, CGA. High concentrations of CGA and relatively low pH occur in the vacuoles of plant cells. Transient assays demonstrated that tomato HQT localizes to the vacuole as well as to the cytoplasm of plant cells, supporting the idea that in this species, the enzyme catalyzes different reactions in two subcellular compartments.
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