ArticleScience (New York, N.Y.)2014
Spatial organization of cytokinesis signaling reconstituted in a cell-free system.
Article in Science (New York, N.Y.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed, 131 citations in OpenAlex.
- Membrane composition-dependent patterning of Rho and F-actin in an artificial cell cortex.Molecular biology of the cell · 2026Article
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- Article
- A mechanical ratchet drives unilateral cytokinesis.Nature · 2026Article
- How does cytoplasmic crowding affect reaction rates?Molecular cell · 2026Review
- An acentrosomal aster with atypical microtubule polarity recruits cytokinesis signals to its center in Xenopus egg extracts.Journal of cell science · 2025Article
- Force balance of opposing diffusive motors generates polarity-sorted microtubule patterns.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Involvement of kinesins in skeletal dysplasia: a review.American journal of physiology. Cell physiology · 2024Review
- Enucleation of theLife science alliance · 2024Article
- Centralspindlin-mediated transport of RhoGEF positions the cleavage plane for cytokinesis.Science signaling · 2023Article
- Article
- Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts.Communications biology · 2022Article
- Article
- Cytoplasmic organization promotes protein diffusion in Xenopus extracts.Nature communications · 2022Article
- Dynein-dependent collection of membranes defines the architecture and position of microtubule asters in isolated, geometrically confined volumes of cell-free extracts.Molecular biology of the cell · 2022Article
- Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence (TIRF) Microscopy.Journal of visualized experiments : JoVE · 2022Article
- Astral microtubule cross-linking safeguards uniform nuclear distribution in the Drosophila syncytium.The Journal of cell biology · 2022Article
- Rho and F-actin self-organize within an artificial cell cortex.Current biology : CB · 2021Article
- Self-Organization of Cellular Units.Annual review of cell and developmental biology · 2021Review
- Reconstitution of muscle cell microtubule organization in vitro.Cytoskeleton (Hoboken, N.J.) · 2021Article
12 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
During animal cell division, the cleavage furrow is positioned by microtubules that signal to the actin cortex at the cell midplane. We developed a cell-free system to recapitulate cytokinesis signaling using cytoplasmic extract from Xenopus eggs. Microtubules grew out as asters from artificial centrosomes and met to organize antiparallel overlap zones. These zones blocked the interpenetration of neighboring asters and recruited cytokinesis midzone proteins, including the chromosomal passenger complex (CPC) and centralspindlin. The CPC was transported to overlap zones, which required two motor proteins, Kif4A and a Kif20A paralog. Using supported lipid bilayers to mimic the plasma membrane, we observed the recruitment of cleavage furrow markers, including an active RhoA reporter, at microtubule overlaps. This system opens further approaches to understanding the biophysics of cytokinesis signaling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.