ArticleApplied microbiology and biotechnology2015
Identification of a small molecule signaling factor that regulates the biosynthesis of the antifungal polycyclic tetramate macrolactam HSAF in Lysobacter enzymogenes.
Article in Applied microbiology and biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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36 citing papers in PubMed, 64 citations in OpenAlex.
- Old role with new feature: T2SS ATPase as a cyclic-di-GMP receptor to regulate antibiotic production.Applied and environmental microbiology · 2024Article
- Quorum quenching by a type IVA secretion system effector.The ISME journal · 2023Article
- A Novel and Efficient Platform for Discovering Noncanonical Quorum-Quenching Proteins.Microbiology spectrum · 2023Article
- Characterization ofAIMS microbiology · 2023Article
- Biofilm formation and inhibition mediated by bacterial quorum sensing.Applied microbiology and biotechnology · 2022Review
- Engineered bacteria as drug delivery vehicles: Principles and prospects.Engineering microbiology · 2022Review
- Diguanylate Cyclase and Phosphodiesterase Interact To Maintain the Specificity of Cyclic di-GMP Signaling in the Regulation of Antibiotic Synthesis in Lysobacter enzymogenes.Applied and environmental microbiology · 2022Article
- Comparative genomics provides insights into the potential biocontrol mechanism of twoFrontiers in microbiology · 2022Article
- The predatory soil bacterium Lysobacter reprograms quorum sensing system to regulate antifungal antibiotic production in a cyclic-di-GMP-independent manner.Communications biology · 2021Article
- Coordinated control of the type IV pili and c-di-GMP-dependent antifungal antibiotic production in Lysobacter by the response regulator PilR.Molecular plant pathology · 2021Article
- Bacterial quorum sensing quenching activity ofComputational and structural biotechnology journal · 2021Article
- The Perception of Rhizosphere Bacterial Communication Signals Leads to Transcriptome Reprogramming inFrontiers in microbiology · 2021Article
- Clp is a "busy" transcription factor in the bacterial warrior,Computational and structural biotechnology journal · 2021Review
- LeTetR Positively Regulates 3-Hydroxylation of the Antifungal HSAF and Its Analogs inMolecules (Basel, Switzerland) · 2020Article
- Two Functional Fatty Acyl Coenzyme A Ligases Affect Free Fatty Acid Metabolism To Block Biosynthesis of an Antifungal Antibiotic in Lysobacter enzymogenes.Applied and environmental microbiology · 2020Article
- FilamentousFrontiers in microbiology · 2020Article
- Interspecies and Intraspecies Signals Synergistically Regulate Lysobacter enzymogenes Twitching Motility.Applied and environmental microbiology · 2019Article
- Indole Reverses Intrinsic Antibiotic Resistance by Activating a Novel Dual-Function Importer.mBio · 2019Article
- The AHL Quorum-Sensing System Negatively Regulates Growth and Autolysis inFrontiers in microbiology · 2019Article
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
Lysobacter species are emerging as new sources of antibiotics. The regulation of these antibiotics is not well understood. Here, we identified a small molecule metabolite (LeDSF3) that regulates the biosynthesis of the antifungal antibiotic heat-stable antifungal factor (HSAF), a polycyclic tetramate macrolactam with a structure and mode of action distinct from the existing antifungal drugs. LeDSF3 was isolated from the culture broth of Lysobacter enzymogenes, and its chemical structure was established by NMR and MS. The purified compound induced green fluorescence in a reporter strain of Xanthomonas campestris, which contained a gfp gene under the control of a diffusible signaling factor (DSF)-inducible promoter. Exogenous addition of LeDSF3 in L. enzymogenes cultures significantly increased the HSAF yield, the transcription of HSAF biosynthetic genes, and the antifungal activity of the organism. The LeDSF3-regulated HSAF production is dependent on the two-component regulatory system RpfC/RpfG. Moreover, LeDSF3 upregulated the expression of the global regulator cAMP receptor-like protein (Clp). The disruption of clp led to no HSAF production. Together, the results show that LeDSF3 is a fatty acid-derived, diffusible signaling factor positively regulating HSAF biosynthesis and that the signaling is mediated by the RfpC/RpfG-Clp pathway. These findings may facilitate the antibiotic production through applied genetics and molecular biotechnology in Lysobacter, a group of ubiquitous yet underexplored microorganisms.
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