ArticleApplied microbiology and biotechnology2015
Biosynthetic preparation of selectively deuterated phosphatidylcholine in genetically modified Escherichia coli.
Article in Applied microbiology and biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 35 citations in OpenAlex.
- Controlled Deuteration of Phosphatidylcholine Biosynthetically Produced from EngineeredACS omega · 2026Article
- The impact of folding modes and deuteration on the atomic resolution structure of hen egg-white lysozyme.Acta crystallographica. Section D, Structural biology · 2021Article
- Recombinant and endogenous ways to produce methylated phospholipids in Escherichia coli.Applied microbiology and biotechnology · 2021Article
- Lipids and Lipid Derivatives for RNA Delivery.Chemical reviews · 2021Review
- Article
- Interdomain Flexibility within NADPH Oxidase Suggested by SANS Using LMNG Stealth Carrier.Biophysical journal · 2020Article
- Examining Membrane Proteins by Neutron Scattering.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Time-resolved small-angle neutron scattering as a probe for the dynamics of lipid exchange between human lipoproteins and naturally derived membranes.Scientific reports · 2019Article
- The Production of Matchout-Deuterated Cholesterol and the Study of Bilayer-Cholesterol Interactions.Scientific reports · 2019Article
- Structural basis for activation of plasma-membrane CaCommunications biology · 2018Article
- Nanodiscs in Membrane Biochemistry and Biophysics.Chemical reviews · 2017Review
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Authors and funding
11 authors at 4 institutions in 4 countries.
Funding
Abstract
Phosphatidylcholine (PC) is a major component of eukaryotic cell membranes and one of the most commonly used phospholipids for reconstitution of membrane proteins into carrier systems such as lipid vesicles, micelles and nanodiscs. Selectively deuterated versions of this lipid have many applications, especially in structural studies using techniques such as NMR, neutron reflectivity and small-angle neutron scattering. Here we present a comprehensive study of selective deuteration of phosphatidylcholine through biosynthesis in a genetically modified strain of Escherichia coli. By carefully tuning the deuteration level in E. coli growth media and varying the deuteration of supplemented carbon sources, we show that it is possible to achieve a controlled deuteration for three distinct parts of the PC lipid molecule, namely the (a) lipid head group, (b) glycerol backbone and (c) fatty acyl tail. This biosynthetic approach paves the way for the synthesis of specifically deuterated, physiologically relevant phospholipid species which remain difficult to obtain through standard chemical synthesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.