ArticleScientific reports2014
Comprehensive quantification of triacylglycerols in soybean seeds by electrospray ionization mass spectrometry with multiple neutral loss scans.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Recent Advances in the Characterization of Food Biomacromolecules: Polysaccharide, Protein, and Lipid.Food science & nutrition · 2025Review
- Fast autoxidation of unsaturated lipid films on indoor surfaces.Nature communications · 2025Article
- Evaluation of the residual feed intake on carcass and meat quality traits of Nellore bulls: a biochemical and molecular approach.Journal of animal science · 2025Article
- Lipidomic Profiling and Storage-Induced Changes in Cassava Flour Using LC-MS/MS.Foods (Basel, Switzerland) · 2024Article
- Article
- Shotgun-Based Mass Spectrometry Analysis of Phospholipid and Triacylglycerol Molecular Species and Eicosanoids in Salmon Muscle Tissue on Feeding Microbial Oil.Marine drugs · 2023Article
- A Critical Overview of HPLC-MS-Based Lipidomics in Determining Triacylglycerol and Phospholipid in Foods.Foods (Basel, Switzerland) · 2023Review
- Targeted Lipidomics Analysis of Adipose and Skeletal Muscle Tissues by Multiple Reaction Monitoring Profiling.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Combined Transcriptome and Lipidomic Analyses of Lipid Biosynthesis inLife (Basel, Switzerland) · 2021Article
- Shotgun lipidomics of liver and brain tissue of Alzheimer's disease model mice treated with acitretin.Scientific reports · 2021Article
- Genotyping and lipid profiling of 601 cultivated sunflower lines reveals novel genetic determinants of oil fatty acid content.BMC genomics · 2021Article
- Lipidomic profiling of the developing kernel clarifies the lipid metabolism of Paeonia ostii.Scientific reports · 2021Article
- TAILOR-MS, a Python Package that Deciphers Complex Triacylglycerol Fatty Acyl Structures: Applications for Bovine Milk and Infant Formulas.Analytical chemistry · 2021Article
- Generative Algorithm for Molecular Graphs Uncovers Products of Oil Oxidation.Journal of chemical information and modeling · 2021Article
- Lipid profiling suggests species specificity and minimal seasonal variation in Pacific Green and Hawksbill Turtle plasma.PloS one · 2021Article
- Evaluation of lipid quantification accuracy using HILIC and RPLC MS on the example of NIST® SRM® 1950 metabolites in human plasma.Analytical and bioanalytical chemistry · 2020Article
- An efficient and comprehensive plant glycerolipids analysis approach based on high-performance liquid chromatography-quadrupole time-of-flight mass spectrometer.Plant direct · 2019Article
- Multiple reaction monitoring (MRM)-profiling with biomarker identification by LC-QTOF to characterize coronary artery disease.The Analyst · 2018Article
- Mass spectrometry-based shotgun lipidomics - a critical review from the technical point of view.Analytical and bioanalytical chemistry · 2018Review
- Profiling of epidermal lipids in a mouse model of dermatitis: Identification of potential biomarkers.PloS one · 2018Article
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Authors and funding
3 authors.
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Abstract
Soybean seeds are an important source of vegetable oil and biomaterials. The content of individual triacylglycerol species (TAG) in soybean seeds is difficult to quantify in an accurate and rapid way. The present study establishes an approach to quantify TAG species in soybean seeds utilizing an electrospray ionization tandem mass spectrometry with multiple neutral loss scans. Ten neutral loss scans were performed to detect the fatty acyl chains of TAG, including palmitic (P, 16:0), linolenic (Ln, 18:3), linoleic (L, 18:2), oleic (O, 18:1), stearic (S, 18:0), eicosadienoic (20:2), gadoleic (20:1), arachidic (20:0), erucic (22:1), and behenic (22:0). The abundance of ten fatty acyl chains at 46 TAG masses (mass-to-charge ratio, m/z) were determined after isotopic deconvolution and correction by adjustment factors at each TAG mass. The direct sample infusion and multiple internal standards correction allowed a rapid and accurate quantification of TAG species. Ninety-three TAG species were resolved and their levels were determined. The most abundant TAG species were LLL, OLL, LLLn, PLL, OLLn, OOL, POL, and SLL. Many new species were detected and quantified. This shotgun lipidomics approach should facilitate the study of TAG metabolism and genetic breeding of soybean seeds for desirable TAG content and composition.
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