Evidence map›Paper›PMID 25300353›Full record

ArticleFolia microbiologica2015

Evaluation of biochemical and molecular methods for Lactobacillus reuteri strains differentiation.

Bilková Andrea, Kiňová Sepová Hana, Dubničková Martina, Májeková Hyacinta, Bilka František

Abstract readComparative StudyEvaluation Study
PubMed Publisher
In one paragraph

Article in Folia microbiologica, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Molecular characterization of microbial contaminants isolated from Umbilical Cord Blood Units for transplant.The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Bilková AndreaDepartment of Cell and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University in Bratislava, Bratislava, Slovak Republic, bilkova@fpharm.uniba.sk.
Kiňová Sepová Hana
Dubničková Martina
Májeková Hyacinta
Bilka František

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Several biochemical and molecular methods were used for discrimination of four Lactobacillus reuteri strains isolated from goatling and lamb stomach mucosa. Internal transcribed spacer (ITS)-PCR method and protein analysis by SDS-PAGE and MALDI-TOF showed to be suitable for strain discrimination whereas ITS-PCR/RFLP and enterobacterial repetitive intergenic consensus (ERIC)-PCR were not strain specific. The used methods differentiated tested strains into distinct groups; however, the location of strains in groups varied. Consistency in results was observed in the case of L. reuteri E and L. reuteri KO4m that were clustered into the same groups using all techniques, except of MALDI-TOF MS. The last one grouped goatling strains and lamb isolate into separate clusters. All investigated methods, except of ITS-PCR/RFLP and ERIC-PCR, were assessed as appropriate for distinguishing of L. reuteri strains.

Indexed as

AnimalsBacterial ProteinsBacteriological TechniquesCluster AnalysisDNA, BacterialDNA, Ribosomal SpacerElectrophoresis, Polyacrylamide GelGastric MucosaGoatsLimosilactobacillus reuteriPolymerase Chain ReactionReproducibility of ResultsSheepSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationBacterial ProteinsDNA, BacterialDNA, Ribosomal Spacer

Identifiers

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.