ArticleScientific reports2014
A Halloween gene noppera-bo encodes a glutathione S-transferase essential for ecdysteroid biosynthesis via regulating the behaviour of cholesterol in Drosophila.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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46 citing papers in PubMed, 101 citations in OpenAlex.
- RNAi-Mediated Knockdown of Halloween Genes in Lygus hesperus Has Varied Effects on Oogenesis and Fertility.Archives of insect biochemistry and physiology · 2026Article
- Genetic and Molecular Regulation of Ecdysone Biosynthesis During Insect Metamorphosis.Insects · 2026Review
- AZoological research · 2026Article
- Pleiotropic Cholesterol Signaling inMetabolites · 2026Review
- Photoperiodic plasticity of pigment-dispersing factor immunoreactive fibers projecting toward prothoracicotropic hormone neurons in flesh fly Sarcophaga similis larvae.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2025Article
- Article
- Developmental analysis of Spalt function in the Drosophila prothoracic gland.Development (Cambridge, England) · 2024Article
- Structural and Thermodynamic Insights into Dimerization Interfaces of Drosophila Glutathione Transferases.Biomolecules · 2024Article
- Genetic Mapping and Phenotypic Analysis ofmicroPublication biology · 2024Article
- Versatility of Glutathione Transferase Proteins.Biomolecules · 2023Article
- Sterol Regulation of Development and 20-Hydroxyecdysone Biosynthetic and Signaling Genes inCells · 2023Article
- Potent GST Ketosteroid Isomerase Activity Relevant to Ecdysteroidogenesis in the Malaria VectorBiomolecules · 2023Article
- Compounds Inhibiting Noppera-bo, a GlutathioneBiomolecules · 2023Review
- Role of Insect and Mammal Glutathione Transferases in Chemoperception.Biomolecules · 2023Review
- cis-Decalin-containing tetramic acids as inhibitors of insect steroidogenic glutathione S-transferase Noppera-bo.PloS one · 2023Article
- Article
- Cyromazine Effects the Reproduction ofInsects · 2022Article
- Molecular action of larvicidal flavonoids on ecdysteroidogenic glutathione S-transferase Noppera-bo in Aedes aegypti.BMC biology · 2022Article
- Transcriptional Regulators of Ecdysteroid Biosynthetic Enzymes and Their Roles in Insect Development.Frontiers in physiology · 2022Review
- New ways and new hopes for IGR development.Journal of pesticide science · 2021Article
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In insects, the precise timing of moulting and metamorphosis is strictly guided by ecdysteroids that are synthesised from dietary cholesterol in the prothoracic gland (PG). In the past decade, several ecdysteroidogenic enzymes, some of which are encoded by the Halloween genes, have been identified and characterised. Here, we report a novel Halloween gene, noppera-bo (nobo), that encodes a member of the glutathione S-transferase family. nobo was identified as a gene that is predominantly expressed in the PG of the fruit fly Drosophila melanogaster. We generated a nobo knock-out mutant, which displayed embryonic lethality and a naked cuticle structure. These phenotypes are typical for Halloween mutants showing embryonic ecdysteroid deficiency. In addition, the PG-specific nobo knock-down larvae displayed an arrested phenotype and reduced 20-hydroxyecdysone (20E) titres. Importantly, both embryonic and larval phenotypes were rescued by the administration of 20E or cholesterol. We also confirm that PG cells in nobo loss-of-function larvae abnormally accumulate cholesterol. Considering that cholesterol is the most upstream material for ecdysteroid biosynthesis in the PG, our results raise the possibility that nobo plays a crucial role in regulating the behaviour of cholesterol in steroid biosynthesis in insects.
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