ArticleTissue engineering. Part A2015
The influence of stromal cells on the pigmentation of tissue-engineered dermo-epidermal skin grafts.
Article in Tissue engineering. Part A, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 29 citations in OpenAlex.
- Isolation and Culture of Human Dermal Fibroblasts.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Standard Preparation Protocol of Human Skin and Skin Substitute Samples for Transmission Electron Microscopy.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Isolation and Culture of Human Dermal Fibroblasts.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Using Integra for Reconstruction of Facial Defects after Mohs Micrographic Surgery.Plastic and reconstructive surgery. Global open · 2023Article
- Combining bioengineered human skin with bioprinted cartilage for ear reconstruction.Science advances · 2023Article
- Effects of an Adipose Mesenchymal Stem Cell-Derived Conditioned medium and TGF-β1 on Human Keratinocytes In Vitro.International journal of molecular sciences · 2023Article
- Article
- Human fetal skin derived merkel cells display distinctive characteristicsFrontiers in bioengineering and biotechnology · 2022Article
- Impact of human mesenchymal cells of different body site origins on the maturation of dermo-epidermal skin substitutes.Pediatric surgery international · 2019Article
- Characterization of the human ridged and non-ridged skin: a comprehensive histological, histochemical and immunohistochemical analysis.Histochemistry and cell biology · 2019Article
- The expression pattern of keratin 24 in tissue-engineered dermo-epidermal human skin substitutes in an in vivo model.Pediatric surgery international · 2018Article
- Review
- Myelinated and unmyelinated nerve fibers reinnervate tissue-engineered dermo-epidermal human skin analogs in an in vivo model.Pediatric surgery international · 2016Article
- Bioprinting and plastic compression of large pigmented and vascularized human dermo-epidermal skin substitutes by means of a new robotic platform.Journal of tissue engineeringArticle
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It has been shown in vitro that melanocyte proliferation and function in palmoplantar skin is regulated by mesenchymal factors derived from fibroblasts. In this study, we investigated in vivo the influence of mesenchymal-epithelial interactions in human tissue-engineered skin substitutes reconstructed from palmar- and nonpalmoplantar-derived fibroblasts. Tissue-engineered dermo-epidermal analogs based on collagen type I hydrogels were populated with either human palmar or nonpalmoplantar fibroblasts and seeded with human nonpalmoplantar-derived melanocytes and keratinocytes. These skin substitutes were transplanted onto full-thickness skin wounds of immunoincompetent rats. Four weeks after transplantation the development of skin color was measured and grafts were excised and analyzed with regard to epidermal characteristics, in particular melanocyte number and function. Skin substitutes containing palmar-derived fibroblasts in comparison to nonpalmoplantar-derived fibroblasts showed (a) a significantly lighter pigmentation; (b) a reduced amount of epidermal melanin granules; and (c) a distinct melanosome expression. However, the number of melanocytes in the basal layer remained similar in both transplantation groups. These findings demonstrate that human palmar fibroblasts regulate the function of melanocytes in human pigmented dermo-epidermal skin substitutes after transplantation, whereas the number of melanocytes remains constant. This underscores the influence of site-specific stromal cells and their importance when constructing skin substitutes for clinical application.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.