ArticlePLoS pathogens2014
Systematic analysis of Zn2Cys6 transcription factors required for development and pathogenicity by high-throughput gene knockout in the rice blast fungus.
Article in PLoS pathogens, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 102 papers.
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102 citing papers in PubMed, 200 citations in OpenAlex.
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- Endophyte Acrocalymma vagum establishes the holobiont with rice to attract beneficial microorganisms and promote disease resistance.Journal of advanced research · 2026Article
- Integrative perspectives on glycosylation networks in fungi and oomycetes.Journal of microbiology (Seoul, Korea) · 2025Review
- MoMkt1, a member of XPG/RAD2 nuclease family, regulates development and pathogenicity inVirulence · 2025Article
- MoSec13 combined with MoGcn5b modulates MoAtg8 acetylation and regulates autophagy inAutophagy · 2025Article
- Rapid filamentous fungi gene knockout identification through high-throughput droplet microfluidics.Analytica chimica acta · 2025Article
- Golgin Protein MoCoy1 Mediates Retrograde Trafficking From the Golgi to the ER, Regulating Fungal Development and Pathogenicity in Magnaporthe oryzae.Molecular plant pathology · 2025Article
- A multilayered regulatory network mediated by protein phosphatase 4 controls carbon catabolite repression and de-repression in Magnaporthe oryzae.Communications biology · 2025Article
- Harnessing the Tet-Off system for effective gene silencing inFrontiers in plant science · 2025Article
- MaAzaR Influences Virulence ofJournal of fungi (Basel, Switzerland) · 2024Article
- Genome sequencing of Elaeocarpus spp. stem blight pathogen Pseudocryphonectria elaeocarpicola reveals potential adaptations to colonize woody bark.BMC genomics · 2024Article
- Functional Characterization of the Transcription Factor GeneJournal of fungi (Basel, Switzerland) · 2024Article
- Dihydroorotase MoPyr4 is required for development, pathogenicity, and autophagy in rice blast fungus.Cell communication and signaling : CCS · 2024Article
- Csn5 inhibits autophagy by regulating the ubiquitination of Atg6 and Tor to mediate the pathogenicity of Magnaporthe oryzae.Cell communication and signaling : CCS · 2024Article
42 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Because of great challenges and workload in deleting genes on a large scale, the functions of most genes in pathogenic fungi are still unclear. In this study, we developed a high-throughput gene knockout system using a novel yeast-Escherichia-Agrobacterium shuttle vector, pKO1B, in the rice blast fungus Magnaporthe oryzae. Using this method, we deleted 104 fungal-specific Zn(2)Cys(6) transcription factor (TF) genes in M. oryzae. We then analyzed the phenotypes of these mutants with regard to growth, asexual and infection-related development, pathogenesis, and 9 abiotic stresses. The resulting data provide new insights into how this rice pathogen of global significance regulates important traits in the infection cycle through Zn(2)Cys(6)TF genes. A large variation in biological functions of Zn(2)Cys(6)TF genes was observed under the conditions tested. Sixty-one of 104 Zn(2)Cys(6) TF genes were found to be required for fungal development. In-depth analysis of TF genes revealed that TF genes involved in pathogenicity frequently tend to function in multiple development stages, and disclosed many highly conserved but unidentified functional TF genes of importance in the fungal kingdom. We further found that the virulence-required TF genes GPF1 and CNF2 have similar regulation mechanisms in the gene expression involved in pathogenicity. These experimental validations clearly demonstrated the value of a high-throughput gene knockout system in understanding the biological functions of genes on a genome scale in fungi, and provided a solid foundation for elucidating the gene expression network that regulates the development and pathogenicity of M. oryzae.
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