ArticlePloS one2014
Stimulatory effects of polysaccharide fraction from Solanum nigrum on RAW 264.7 murine macrophage cells.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 48 citations in OpenAlex.
- Effect of Bean-Derived Soluble Dietary Fibers on Macrophage Function In Vitro.Foods (Basel, Switzerland) · 2026Article
- Research Progress on the Anticancer Activity of Plant Polysaccharides.Recent patents on anti-cancer drug discovery · 2024Review
- Understanding immune-modulatory efficacy in vitro.Chemico-biological interactions · 2022Review
- Free Radical Scavenging Capacities and Alleviating Actions of Polysaccharides Extract ofAdvances in pharmacological and pharmaceutical sciences · 2021Article
- Article
- In Vivo and In Vitro Study of Immunostimulation byMolecules (Basel, Switzerland) · 2019Article
- Water Soluble Antioxidative Crude Polysaccharide FromFrontiers in pharmacology · 2018Article
- Introducing a novel mushroom from mycophagy community with emphasis on biomedical potency.PloS one · 2017Article
- Effects of Rhodomyrtus tomentosa Extract on Killing Activity of Human Neutrophils and Membrane Integrity of Enterohaemorrhagic Escherichia coli O157:H7.Molecules (Basel, Switzerland) · 2016Article
- Effect and Molecular Mechanisms of Traditional Chinese Medicine on Regulating Tumor Immunosuppressive Microenvironment.BioMed research international · 2015Review
- Comparative Analysis of Transcriptomes of Macrophage Revealing the Mechanism of the Immunoregulatory Activities of a Novel Polysaccharide Isolated fromPharmacognosy magazineArticle
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The polysaccharide fraction from Solanum nigrum Linne has been shown to have antitumor activity by enhancing the CD4+/CD8+ ratio of the T-lymphocyte subpopulation. In this study, we analyzed a polysaccharide extract of S. nigrum to determine its modulating effects on RAW 264.7 murine macrophage cells since macrophages play a key role in inducing both innate and adaptive immune responses. Crude polysaccharide was extracted from the stem of S. nigrum and subjected to ion-exchange chromatography to partially purify the extract. Five polysaccharide fractions were then subjected to a cytotoxicity assay and a nitric oxide production assay. To further analyze the ability of the fractionated polysaccharide extract to activate macrophages, the phagocytosis activity and cytokine production were also measured. The polysaccharide fractions were not cytotoxic, but all of the fractions induced nitric oxide in RAW 264.7 cells. Of the five fractions tested, SN-ppF3 was the least toxic and also induced the greatest amount of nitric oxide, which was comparable to the inducible nitric oxide synthase expression detected in the cell lysate. This fraction also significantly induced phagocytosis activity and stimulated the production of tumor necrosis factor-α and interleukin-6. Our study showed that fraction SN-ppF3 could classically activate macrophages. Macrophage induction may be the manner in which polysaccharides from S. nigrum are able to prevent tumor growth.
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