ArticleAnalytical chemistry2014
Liquid chromatography-selected reaction monitoring (LC-SRM) approach for the separation and quantitation of sialylated N-glycans linkage isomers.
Article in Analytical chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 65 citations in OpenAlex.
- Glycosylation in kidney diseases.Precision clinical medicine · 2025Review
- Characterizing Glycosylation of Adeno-Associated Virus Serotype 9 Capsid Proteins Generated from HEK293 Cells through Glycopeptide Mapping and Released Glycan Analysis.Microorganisms · 2024Article
- Targeted Analysis of Permethylated N-Glycans Using MRM/PRM Approaches.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Comprehensive N-Glycan Mapping using Parallel Reaction Monitoring LC-MS/MS.Pharmaceutical research · 2023Article
- Highly-sensitive label-free deep profiling of N-glycans released from biomedically-relevant samples.Nature communications · 2023Article
- Applications and continued evolution of glycan imaging mass spectrometry.Mass spectrometry reviews · 2023Review
- An Efficient and EconomicalMetabolites · 2022Article
- Quantitative clinical glycomics strategies: A guide for selecting the best analysis approach.Mass spectrometry reviews · 2022Review
- Determination of Sialic Acid Isomers from ReleasedAnalytical chemistry · 2022Article
- Review
- Recent Advances in Analytical Approaches for Glycan and Glycopeptide Quantitation.Molecular & cellular proteomics : MCP · 2021Review
- Comparison of Different HILIC Stationary Phases in the Separation of Hemopexin and Immunoglobulin G Glycopeptides and Their Isomers.Molecules (Basel, Switzerland) · 2020Article
- Glycan-specific precipitation of glycopeptides in high organic content sample solvents used in HILIC.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2020Article
- Separation of Sialylated Glycan Isomers by Differential Mobility Spectrometry.Analytical chemistry · 2019Article
- Sialic acid derivatization for glycan analysis by mass spectrometry.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2019Review
- Predicting the HILIC Retention Behavior of the N-Linked Glycopeptides Produced by Trypsin Digestion of Immunoglobulin Gs (IgGs).Journal of biomolecular techniques : JBT · 2018Article
- Hydrophilic interaction liquid chromatography in the separation of glycopeptides and their isomers.Analytical and bioanalytical chemistry · 2018Article
- Direct comparison of derivatization strategies for LC-MS/MS analysis of N-glycans.The Analyst · 2017Article
- Characterization of isomeric glycan structures by LC-MS/MS.Electrophoresis · 2017Review
- LC-MS/MS isomeric profiling of permethylated N-glycans derived from serum haptoglobin of hepatocellular carcinoma (HCC) and cirrhotic patients.Electrophoresis · 2017Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The study of N-linked glycans is among the most challenging bioanalytical tasks because of their complexity and variety. The presence of glycoform families that differ only in branching and/or linkage position makes the identification and quantitation of individual glycans exceedingly difficult. Quantitation of these individual glycans is important because changes in the abundance of these isomers are often associated with significant biomedical events. For instance, previous studies have shown that the ratio of α2-3 to α2-6 linked sialic acid (SA) plays an important role in cancer biology. Consequently, quantitative methods to detect alterations in the ratios of glycans based on their SA linkages could serve as a diagnostic tool in oncology, yet traditional glycomic profiling cannot readily differentiate between these linkage isomers. Here, we present a liquid chromatography-selected reaction monitoring (LC-SRM) approach that we demonstrate is capable of quantitating the individual SA linkage isomers. The LC method is capable of separating sialylated N-glycan isomers differing in α2-3 and α2-6 linkages using a novel superficially porous particle (Fused-Core) Penta-HILIC (hydrophilic interaction liquid chromatography) column. SRM detection provides the relative quantitation of each SA linkage isomer, and minimizes interferences from coeluting glycans that are problematic for UV/Fluorescence based quantitation. With our approach, the relative quantitation of each SA linkage isomer is obtained from a straightforward liquid chromatography-mass spectrometry (LC-MS) experiment.
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