ArticleAmerican journal of physiology. Renal physiology2014
Local pH domains regulate NHE3-mediated Na⁺ reabsorption in the renal proximal tubule.
Article in American journal of physiology. Renal physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 47 citations in OpenAlex.
- Sexual dimorphic pattern of renal transporters and channels in spontaneously hypertensive rats.American journal of physiology. Cell physiology · 2026Article
- Unique Role of Proximal Tubule Dipeptidyl Peptidase 4 on Blood Pressure, Renal Sodium Handling, and NaActa physiologica (Oxford, England) · 2025Article
- Epac2 Deficiency Compromises Adaptation to Dietary Acidification by Decreasing H+ Transport in the Renal Nephron.Function (Oxford, England) · 2025Article
- Effect of sex chromosome complement versus gonadal hormones on abundance of renal transporters.American journal of physiology. Renal physiology · 2025Article
- How the kidney regulates magnesium: a modelling study.Royal Society open science · 2024Article
- Physiological Mechanisms of Dietary Salt Sensing in the Brain, Kidney, and Gastrointestinal Tract.Hypertension (Dallas, Tex. : 1979) · 2024Review
- Sex differences in renal transporters: assessment and functional consequences.Nature reviews. Nephrology · 2024Review
- Angiotensin II hypertension along the female rat tubule: predicted impact on coupled transport of NaAmerican journal of physiology. Renal physiology · 2023Article
- High dietary KAmerican journal of physiology. Renal physiology · 2023Article
- Transport Turnover Rates for Human OCT2 and MATE1 Expressed in Chinese Hamster Ovary Cells.International journal of molecular sciences · 2022Article
- Sex and species differences in epithelial transport in rat and mouse kidneys: Modeling and analysis.Frontiers in physiology · 2022Article
- Sex-specific adaptations to high-salt diet preserve electrolyte homeostasis with distinct sodium transporter profiles.American journal of physiology. Cell physiology · 2021Article
- Sex differences in solute and water handling in the human kidney: Modeling and functional implications.iScience · 2021Article
- A role for tubular NaAmerican journal of physiology. Renal physiology · 2020Article
- Sex differences in solute transport along the nephrons: effects of NaAmerican journal of physiology. Renal physiology · 2020Article
- Impact of angiotensin II-mediated stimulation of sodium transporters in the nephron assessed by computational modeling.American journal of physiology. Renal physiology · 2019Article
- Functional implications of the sex differences in transporter abundance along the rat nephron: modeling and analysis.American journal of physiology. Renal physiology · 2019Article
- Renal secretion of hydrochlorothiazide involves organic anion transporter 1/3, organic cation transporter 2, and multidrug and toxin extrusion protein 2-K.American journal of physiology. Renal physiology · 2019Article
- Understanding sex differences in long-term blood pressure regulation: insights from experimental studies and computational modeling.American journal of physiology. Heart and circulatory physiology · 2019Article
- Impaired pressure natriuresis and non-dipping blood pressure in rats with early type 1 diabetes mellitus.The Journal of physiology · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
The proximal tubule Na(+)/H(+) exchanger 3 (NHE3), located in the apical dense microvilli (brush border), plays a major role in the reabsorption of NaCl and water in the renal proximal tubule. In response to a rise in blood pressure NHE3 redistributes in the plane of the plasma membrane to the base of the brush border, where NHE3 activity is reduced. This NHE3 redistribution is assumed to provoke pressure natriuresis; however, it is unclear how NHE3 redistribution per se reduces NHE3 activity. To investigate if the distribution of NHE3 in the brush border can change the reabsorption rate, we constructed a spatiotemporal mathematical model of NHE3-mediated Na(+) reabsorption across a proximal tubule cell and compared the model results with in vivo experiments in rats. The model predicts that when NHE3 is localized exclusively at the base of the brush border, it creates local pH microdomains that reduce NHE3 activity by >30%. We tested the model's prediction experimentally: the rat kidney cortex was loaded with the pH-sensitive fluorescent dye BCECF, and cells of the proximal tubule were imaged in vivo using confocal fluorescence microscopy before and after an increase of blood pressure by ∼50 mmHg. The experimental results supported the model by demonstrating that a rise of blood pressure induces the development of pH microdomains near the bottom of the brush border. These local changes in pH reduce NHE3 activity, which may explain the pressure natriuresis response to NHE3 redistribution.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.