ArticleMolecular biology of the cell2014
Rap1-dependent pathways coordinate cytokinesis in Dictyostelium.
Article in Molecular biology of the cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 21 citations in OpenAlex.
- Loss of Copine D Leads to Ras Activation inbioRxiv : the preprint server for biology · 2026Article
- Microtubule dependent sorting of actin-binding proteins in mitosis.Scientific reports · 2024Article
- GRminusRD: A Sensitive Assay to Detect Activation Processes at the Plasma Membrane in Living Cells.Methods in molecular biology (Clifton, N.J.) · 2024Article
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- Short- and long-term memory of moving amoeboid cells.PloS one · 2021Article
- Opposite functions of RapA and RapC in cell adhesion and migration inAnimal cells and systems · 2021Article
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- Role of the small GTPase Rap1 in signal transduction, cell dynamics and bacterial infection.Small GTPases · 2019Review
- Shear force-based genetic screen reveals negative regulators of cell adhesion and protrusive activity.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Comparative Proteomics of Purified Pathogen Vacuoles Correlates Intracellular Replication ofMolecular & cellular proteomics : MCP · 2017Article
- The small GTPases Ras and Rap1 bind to and control TORC2 activity.Scientific reports · 2016Article
- Direct Interaction between TalinB and Rap1 is necessary for adhesion of Dictyostelium cells.BMC cell biology · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytokinesis is the final step of mitosis when a mother cell is separated into two daughter cells. Major cytoskeletal changes are essential for cytokinesis; it is, however, not well understood how the microtubules and actomyosin cytoskeleton are exactly regulated in time and space. In this paper, we show that during the early stages of cytokinesis, in rounded-up Dictyostelium discoideum cells, the small G-protein Rap1 is activated uniformly at the cell cortex. When cells begin to elongate, active Rap1 becomes restricted from the furrow region, where the myosin contractile ring is subsequently formed. In the final stages of cytokinesis, active Rap1 is only present at the cell poles. Mutant cells with decreased Rap1 activation at the poles showed strongly decreased growth rates. Hyperactivation of Rap1 results in severe growth delays and defective spindle formation in adherent cells and cell death in suspension. Furthermore, Rap mutants show aberrant regulation of the actomyosin cytoskeleton, resulting in extended furrow ingression times and asymmetrical cell division. We propose that Rap1 drives cytokinesis progression by coordinating the three major cytoskeletal components: microtubules, actin, and myosin II. Importantly, mutated forms of Rap also affect cytokinesis in other organisms, suggesting a conserved role for Rap in cell division.
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