Evidence map›Paper›PMID 25298373›Full record

ArticleStem cells translational medicine2014

Reprogramming human retinal pigmented epithelial cells to neurons using recombinant proteins.

Qirui Hu, Renwei Chen, Tambet Teesalu, Erkki Ruoslahti, Dennis O Clegg

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells translational medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.4field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 31 citations in OpenAlex.

  1. Review
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  9. Article
  10. Cellular Reprogramming Using Protein and Cell-Penetrating Peptides.International journal of molecular sciences · 2017
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Qirui HuCenter for Stem Cell Biology and Engineering and Center for Nanomedicine and Sanford-Burnham Medical Research Institute, University of California Santa Barbara, Santa Barbara, California, USA; Cancer Center, Sanford-Burnham Medical Research Institute, La Jolla, California, USA.
Renwei ChenCenter for Stem Cell Biology and Engineering and Center for Nanomedicine and Sanford-Burnham Medical Research Institute, University of California Santa Barbara, Santa Barbara, California, USA; Cancer Center, Sanford-Burnham Medical Research Institute, La Jolla, California, USA.
Tambet TeesaluCenter for Stem Cell Biology and Engineering and Center for Nanomedicine and Sanford-Burnham Medical Research Institute, University of California Santa Barbara, Santa Barbara, California, USA; Cancer Center, Sanford-Burnham Medical Research Institute, La Jolla, California, USA.
Erkki RuoslahtiCenter for Stem Cell Biology and Engineering and Center for Nanomedicine and Sanford-Burnham Medical Research Institute, University of California Santa Barbara, Santa Barbara, California, USA; Cancer Center, Sanford-Burnham Medical Research Institute, La Jolla, California, USA.
Dennis O CleggCenter for Stem Cell Biology and Engineering and Center for Nanomedicine and Sanford-Burnham Medical Research Institute, University of California Santa Barbara, Santa Barbara, California, USA; Cancer Center, Sanford-Burnham Medical Research Institute, La Jolla, California, USA clegg@lifesci.ucsb.edu.
Sanford Burnham Prebys Medical Discovery Institute · USUniversity of California, Santa Barbara · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Somatic cells can be reprogrammed to an altered lineage by overexpressing specific transcription factors. To avoid introducing exogenous genetic material into the genome of host cells, cell-penetrating peptides can be used to deliver transcription factors into cells for reprogramming. Position-dependent C-end rule (CendR) cell- and tissue-penetrating peptides provide an alternative to the conventional cell-penetrating peptides, such as polyarginine. In this study, we used a prototypic, already active CendR peptide, RPARPAR, to deliver the transcription factor SOX2 to retinal pigmented epithelial (RPE) cells. We demonstrated that RPE cells can be directly reprogrammed to a neuronal fate by introduction of SOX2. Resulting neuronal cells expressed neuronal marker mRNAs and proteins and downregulated expression of RPE markers. Cells produced extensive neurites and developed synaptic machinery capable of dye uptake after depolarization with potassium. The RPARPAR-mediated delivery of SOX2 alone was sufficient to allow cell lineage reprogramming of both fetal and stem cell-derived RPE cells to become functional neurons.

Indexed as

Cellular ReprogrammingAntigens, DifferentiationHumansNeuritesRetinal Pigment EpitheliumSOXB1 Transcription FactorsAntigens, DifferentiationSOX2 protein, humanSOXB1 Transcription FactorsCell penetrating peptideLineage reprogrammingNeuronProtein transductionRetinal pigmented epithelium

Identifiers

PMID25298373
PMCPMC4250206
OpenAlexW2149475535

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.