ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2015
Cryopreservation of MHC multimers: Recommendations for quality assurance in detection of antigen specific T cells.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- TCR-T cell therapy: current development approaches, preclinical evaluation, and perspectives on regulatory challenges.Journal of translational medicine · 2024Review
- Discovery of HLA-E-Presented Epitopes: MHC-E/Peptide Binding and T-Cell Recognition.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Production of Class II MHC Proteins in Lentiviral Vector-Transduced HEK-293T Cells for Tetramer Staining Reagents.Current protocols · 2021Article
- Integrin Activation Enables Sensitive Detection of Functional CD4Frontiers in immunology · 2021Article
- Optimization in Detection of Antigen-Specific T Cells Through Differentially Labeled MHC Multimers.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2020Article
- A new synthetic toll-like receptor 1/2 ligand is an efficient adjuvant for peptide vaccination in a human volunteer.Journal for immunotherapy of cancer · 2019Article
- Adhering to adhesion: assessing integrin conformation to monitor T cells.Cancer immunology, immunotherapy : CII · 2019Review
- Induction of neoantigen-reactive T cells from healthy donors.Nature protocols · 2019Article
- GαThe Journal of experimental medicine · 2019Article
- Activated integrins identify functional antigen-specific CD8Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- A flexible MHC class I multimer loading system for large-scale detection of antigen-specific T cells.The Journal of experimental medicine · 2018Article
- Automated Analysis of Flow Cytometry Data to Reduce Inter-Lab Variation in the Detection of Major Histocompatibility Complex Multimer-Binding T Cells.Frontiers in immunology · 2017Article
- A Simple and Rapid Method for Quality Control of Major Histocompatibility Complex-Peptide Monomers by Flow Cytometry.Frontiers in immunology · 2017Article
- Personalized peptide vaccine-induced immune response associated with long-term survival of a metastatic cholangiocarcinoma patient.Journal of hepatology · 2016Article
- Impact of curative radiotherapy on the immune status of patients with localized prostate cancer.OncoimmunologyArticle
- The immunopeptidomic landscape of ependymomas provides actionable antigens for T-cell-based immunotherapy.Neuro-oncology advancesArticle
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescence-labeled peptide-MHC class I multimers serve as ideal tools for the detection of antigen-specific T cells by flow cytometry, enabling functional and phenotypical characterization of specific T cells at the single cell level. While this technique offers a number of unique advantages, MHC multimer reagents can be difficult to handle in terms of stability and quality assurance. The stability of a given fluorescence-labeled MHC multimer complex depends on both the stability of the peptide-MHC complex itself and the stability of the fluorochrome. Consequently, stability is difficult to predict and long-term storage is generally not recommended. We investigated here the possibility of cryopreserving MHC multimers, both in-house produced and commercially available, using a wide range of peptide-MHC class I multimers comprising virus and cancer-associated epitopes of different affinities presented by various HLA-class I molecules. Cryopreservation of MHC multimers was feasible for at least 6 months, when they were dissolved in buffer containing 5-16% glycerol (v/v) and 0.5% serum albumin (w/v). The addition of cryoprotectants was tolerated across three different T-cell staining protocols for all fluorescence labels tested (PE, APC, PE-Cy7 and Quantum dots). We propose cryopreservation as an easily implementable method for stable storage of MHC multimers and recommend the use of cryopreservation in long-term immunomonitoring projects, thereby eliminating the variability introduced by different batches and inconsistent stability.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.