ArticleJournal of the Royal Society, Interface2014
Cell morphology and focal adhesion location alters internal cell stress.
Article in Journal of the Royal Society, Interface, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 52 citations in OpenAlex.
- Cell type-specific response to curvature controls tissue growth dynamics in biomaterial pores.Bioactive materials · 2026Article
- Differences and similarities in biophysical and biological characteristics between U87 MG glioblastoma and astrocyte cells.Histochemistry and cell biology · 2024Article
- Impact of ionizing radiation on cell-ECM mechanical crosstalk in breast cancer.Frontiers in bioengineering and biotechnology · 2024Article
- Chitosan-Silica Hybrid Biomaterials for Bone Tissue Engineering: A Comparative Study of Xerogels and Aerogels.Gels (Basel, Switzerland) · 2023Article
- The actin-bundling protein, PLS3, is part of the mechanoresponsive machinery that regulates osteoblast mineralization.Frontiers in cell and developmental biology · 2023Article
- Discrete protein metric (DPM): A new image similarity metric to calculate accuracy of deep learning-generated cell focal adhesion predictions.Micron (Oxford, England : 1993) · 2022Article
- Sticking around: Cell adhesion patterning for energy minimization and substrate mechanosensing.Biophysical journal · 2022Article
- Finite Element Models of Osteocytes and Their Load-Induced Activation.Current osteoporosis reports · 2022Review
- A possible molecular mechanism for mechanotransduction at cellular focal adhesion complexes.Biophysical reports · 2021Article
- In silico stress fibre content affects peak strain in cytoplasm and nucleus but not in the membrane for uniaxial substrate stretch.Medical & biological engineering & computing · 2021Article
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- Stem Cell Mechanobiology and the Role of Biomaterials in Governing Mechanotransduction and Matrix Production for Tissue Regeneration.Frontiers in bioengineering and biotechnology · 2020Review
- A Novel 3D Osteoblast and Osteocyte Model Revealing Changes in Mineralization and Pro-osteoclastogenic Paracrine Signaling During Estrogen Deficiency.Frontiers in bioengineering and biotechnology · 2020Article
- In vivo engineered extracellular matrix scaffolds with instructive niches for oriented tissue regeneration.Nature communications · 2019Article
- Lateral Spacing of TiOMaterials (Basel, Switzerland) · 2019Article
- The Influence of Surface Wettability and Topography on the Bioactivity of TiOMaterials (Basel, Switzerland) · 2019Article
- The Contribution of Magnetic Nanoparticles to Ferrogel Biophysical Properties.Nanomaterials (Basel, Switzerland) · 2019Article
- Influence of Platelet Lysate on 2D and 3D Amniotic Mesenchymal Stem Cell Cultures.Frontiers in bioengineering and biotechnology · 2019Article
- The effects of hydroxyapatite nanoparticles embedded in a MMP-sensitive photoclickable PEG hydrogel on encapsulated MC3T3-E1 pre-osteoblasts.Biomedical materials (Bristol, England) · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Extracellular mechanical cues have been shown to have a profound effect on osteogenic cell behaviour. However, it is not known precisely how these cues alter intracellular mechanics to initiate changes in cell behaviour. In this study, a combination of in vitro culture of MC3T3-E1 cells and finite-element modelling was used to investigate the effects of passive differences in substrate stiffness on intracellular mechanics. Cells on collagen-based substrates were classified based on the presence of cell processes and the dimensions of various cellular features were quantified. Focal adhesion (FA) density was quantified from immunohistochemical staining, while cell and substrate stiffnesses were measured using a live-cell atomic force microscope. Computational models of cell morphologies were developed using an applied contraction of the cell body to simulate active cell contraction. The results showed that FA density is directly related to cell morphology, while the effect of substrate stiffness on internal cell tension was modulated by both cell morphology and FA density, as investigated by varying the number of adhesion sites present in each morphological model. We propose that the cells desire to achieve a homeostatic stress state may play a role in osteogenic cell differentiation in response to extracellular mechanical cues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.