ArticleNature communications2014
STIM1 triggers a gating rearrangement at the extracellular mouth of the ORAI1 channel.
Article in Nature communications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
58 citing papers in PubMed, 81 citations in OpenAlex.
- Mechanistic insights into Orai dynamics during pore opening.Channels (Austin, Tex.) · 2026Review
- STIM-Orai signaling in neurons: integrating calcium dynamics and synaptic plasticity.Cell communication and signaling : CCS · 2026Review
- Unraveling new functions of the Ca2+ sensor STIM1 in cell signaling.Biochemical Society transactions · 2026Review
- BTP2 restricts Tulane virus and human norovirus replication independent of store-operated calcium entry.Journal of virology · 2025Article
- House dust mite allergens, store-operated CaThe Journal of physiology · 2024Review
- Synthetic Biology Meets CaCells · 2024Review
- Regulatory mechanisms controlling store-operated calcium entry.Frontiers in physiology · 2023Review
- The Role of Lipids in CRAC Channel Function.Biomolecules · 2022Review
- Orai1 Boosts SK3 Channel Activation.Cancers · 2021Article
- Regulation of Store-Operated CaCells · 2021Review
- Electrophysiological Properties of Endogenous Single CaInternational journal of molecular sciences · 2021Article
- Endoplasmic Reticulum-Plasma Membrane Contact Sites as an Organizing Principle for Compartmentalized Calcium and cAMP Signaling.International journal of molecular sciences · 2021Review
- Cross-Talk Between the Adenylyl Cyclase/cAMP Pathway and CaReviews of physiology, biochemistry and pharmacology · 2021Review
- The Impact of Mutation L138F/L210F on the Orai Channel: A Molecular Dynamics Simulation Study.Frontiers in molecular biosciences · 2021Article
- Transmembrane Domain 3 (TM3) Governs Orai1 and Orai3 Pore Opening in an Isoform-Specific Manner.Frontiers in cell and developmental biology · 2021Article
- Molecular Choreography and Structure of CaMembranes · 2020Review
- Cryo-EM structure of the calcium release-activated calcium channel Orai in an open conformation.eLife · 2020Article
- The Intricate Coupling Between STIM Proteins and Orai Channels.Current opinion in physiology · 2020Article
- Structure and Reconstitution of an MCU-EMRE Mitochondrial CaJournal of molecular biology · 2020Article
- STIM calcium sensing and conformational change.The Journal of physiology · 2020Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The ER-resident regulatory protein STIM1 triggers store-operated Ca(2+) entry by direct interaction with the plasma membrane Ca(2+) channel ORAI1. The mechanism of channel gating remains undefined. Here we establish that STIM1 gates the purified recombinant ORAI1 channel in vitro, and use Tb(3+) luminescence and, separately, disulfide crosslinking to probe movements of the pore-lining helices. We show that interaction of STIM1 with the cytoplasmic face of the human ORAI1 channel elicits a conformational change near the external entrance to the pore, detectable at the pore Ca(2+)-binding residue E106 and the adjacent pore-lining residue V102. We demonstrate that a short nonpolar segment of the pore including V102 forms a barrier to ion flux in the closed channel, implicating the STIM1-dependent movement in channel gating. Our data explain the close coupling between ORAI1 channel gating and ion selectivity, and open a new avenue to dissect the gating, modulation and inactivation of ORAI-family channels.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.