ArticleBiophysical journal2014
Imaging GFP-based reporters in neurons with multiwavelength optogenetic control.
Article in Biophysical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 44 citations in OpenAlex.
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- Imaging of mitochondrial matrix pH dynamics reveals a functional interaction between the ADP/ATP carrier and ATP synthase to regulate HPharmacological research · 2025Article
- All-optical mapping of CaCell calcium · 2025Article
- Sensing a rainbow of colors: algal photoreceptors.Frontiers in plant science · 2025Review
- Towards translational optogenetics.Nature biomedical engineering · 2023Review
- Differential Regulation of Prelimbic and Thalamic Transmission to the Basolateral Amygdala by Acetylcholine Receptors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023Article
- All-optical interrogation of neural circuits in behaving mice.Nature protocols · 2022Review
- Molecular Biology of Microbial Rhodopsins.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Optogenetic strategies for high-efficiency all-optical interrogation using blue-light-sensitive opsins.eLife · 2021Article
- Molecular Tuning of the Axonal Mitochondrial CaNeuron · 2020Article
- VAP-SCRN1 interaction regulates dynamic endoplasmic reticulum remodeling and presynaptic function.The EMBO journal · 2019Article
- All-optical crosstalk-free manipulation and readout of Chronos-expressing neurons.Journal of physics D: Applied physics · 2019Article
- Principles of Optogenetic Methods and Their Application to Cardiac Experimental Systems.Frontiers in physiology · 2019Review
- Investigating the feasibility of channelrhodopsin variants for nanoscale optogenetics.Neurophotonics · 2019Article
- Fast, in vivo voltage imaging using a red fluorescent indicator.Nature methods · 2018Article
- Imaging pH Dynamics Simultaneously in Two Cellular Compartments Using a Ratiometric pH-Sensitive Mutant of mCherry.ACS omega · 2018Article
- Two-Photon Bidirectional Control and Imaging of Neuronal Excitability with High Spatial Resolution In Vivo.Cell reports · 2018Article
- Voltage imaging with genetically encoded indicators.Current opinion in chemical biology · 2017Review
- Genetically Targeted All-Optical Electrophysiology with a Transgenic Cre-Dependent Optopatch Mouse.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- All-Optical Interrogation of Neural Circuits.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
To study the impact of neural activity on cellular physiology, one would like to combine precise control of firing patterns with highly sensitive probes of cellular physiology. Light-gated ion channels, e.g., Channelrhodopsin-2, enable precise control of firing patterns; green fluorescent protein-based reporters, e.g., the GCaMP6f Ca(2+) reporter, enable highly sensitive probing of cellular physiology. However, for most actuator-reporter combinations, spectral overlap prevents straightforward combination within a single cell. Here we explore multiwavelength control of channelrhodopsins to circumvent this limitation. The "stoplight" technique described in this article uses channelrhodopsin variants that are opened by blue light and closed by orange light. Cells are illuminated with constant blue light to excite fluorescence of a green fluorescent protein-based reporter. Modulated illumination with orange light negatively regulates activation of the channelrhodopsin. We performed detailed photophysical characterization and kinetic modeling of four candidate stoplight channelrhodopsins. The variant with the highest contrast, sdChR(C138S,E154A), enabled all-optical measurements of activity-induced calcium transients in cultured rat hippocampal neurons, although cell-to-cell variation in expression levels presents a challenge for quantification.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.