ArticleBiophysical journal2014
Combining AFM and acoustic probes to reveal changes in the elastic stiffness tensor of living cells.
Article in Biophysical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Gigantol Preserves Lens Biophysical Homeostasis by Restoring Cytoskeletal Integrity and Membrane Fluidity in a Diabetic Cataract Model.International journal of molecular sciences · 2026Article
- Adhesion response of filopodia to an AFM lateral detachment force and functional changes after centrifugation of cells grown on nanoporous titanium.Materials today. Bio · 2022Article
- Single-cell analysis reveals chemokine-mediated differential regulation of monocyte mechanics.iScience · 2022Article
- Recent Advances on the Model, Measurement Technique, and Application of Single Cell Mechanics.International journal of molecular sciences · 2020Review
- High-throughput cell and spheroid mechanics in virtual fluidic channels.Nature communications · 2020Article
- Nuclear Mechanics within Intact Cells Is Regulated by Cytoskeletal Network and Internal Nanostructures.Small (Weinheim an der Bergstrasse, Germany) · 2020Article
- The Effects ofTissue engineering. Part A · 2020Article
- On-chip surface acoustic wave and micropipette aspiration techniques to assess cell elastic properties.Biomicrofluidics · 2020Article
- Effect of PAK Inhibition on Cell Mechanics Depends on Rac1.Frontiers in cell and developmental biology · 2020Article
- Shear Modulus Measurement by Quantitative Phase Imaging and Correlation with Atomic Force Microscopy.Biophysical journal · 2019Article
- Quantitative atomic force microscopy provides new insight into matrix vesicle mineralization.Archives of biochemistry and biophysics · 2019Article
- Modeling cardiac complexity: Advancements in myocardial models and analytical techniques for physiological investigation and therapeutic developmentAPL bioengineering · 2019Review
- On-chip cell mechanophenotyping using phase modulated surface acoustic wave.Biomicrofluidics · 2019Article
- Characterizing Inner Pressure and Stiffness of Trophoblast and Inner Cell Mass of Blastocysts.Biophysical journal · 2018Article
- Dynamic monitoring of cell mechanical properties using profile microindentation.Scientific reports · 2016Article
- Noncontact three-dimensional mapping of intracellular hydromechanical properties by Brillouin microscopy.Nature methods · 2015Article
- Myosin II Activity Softens Cells in Suspension.Biophysical journal · 2015Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Knowledge of how the elastic stiffness of a cell affects its communication with its environment is of fundamental importance for the understanding of tissue integrity in health and disease. For stiffness measurements, it has been customary to quote a single parameter quantity, e.g., Young's modulus, rather than the minimum of two terms of the stiffness tensor required by elasticity theory. In this study, we use two independent methods (acoustic microscopy and atomic force microscopy nanoindentation) to characterize the elastic properties of a cell and thus determine two independent elastic constants. This allows us to explore in detail how the mechanical properties of cells change in response to signaling pathways that are known to regulate the cell's cytoskeleton. In particular, we demonstrate that altering the tensioning of actin filaments in NIH3T3 cells has a strong influence on the cell's shear modulus but leaves its bulk modulus unchanged. In contrast, altering the polymerization state of actin filaments influences bulk and shear modulus in a similar manner. In addition, we can use the data to directly determine the Poisson ratio of a cell and show that in all cases studied, it is less than, but very close to, 0.5 in value.
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