ArticlePloS one2014
Induction of the viable but nonculturable state of Ralstonia solanacearum by low temperature in the soil microcosm and its resuscitation by catalase.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Unravelling the dual nature of Ralstonia pseudosolanacearum: pathogenicity of type-II viable but nonculturable cells induced by cold and UV-C stress.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Article
- Probiotic Viability Reconsidered: Integrating VBNC Resuscitation and Culture-Independent Methods for Accurate Probiotic Enumeration.Microorganisms · 2025Review
- Time-resolved proteomic profiling of Cupriavidus metallidurans CH34 in the copper-induced viable-but-nonculturable state.Metallomics : integrated biometal science · 2025Article
- Gene expression changes throughout the life cycle allow a bacterial plant pathogen to persist in diverse environmental habitats.PLoS pathogens · 2023Article
- Article
- Comparative Analysis Using Raman Spectroscopy of the Cellular Constituents ofMicroorganisms · 2023Article
- Wake Up! Resuscitation of Viable but Nonculturable Bacteria: Mechanism and Potential Application.Foods (Basel, Switzerland) · 2022Review
- Article
- Biocontrol of bacterial wilt in tomato with a cocktail of lytic bacteriophages.Applied microbiology and biotechnology · 2022Article
- Bacterial hitchhikers derive benefits from fungal housing.Current biology : CB · 2022Article
- Global transcriptome and targeted metabolite analyses of roots reveal different defence mechanisms againstFrontiers in plant science · 2022Article
- Induction and Resuscitation of Viable but NonculturableMicroorganisms · 2021Article
- Persister Cells Form in the Plant PathogenMicroorganisms · 2021Article
- Biocontrol of Bacterial Wilt Disease Through Complex Interaction Between Tomato Plant, Antagonists, the Indigenous Rhizosphere Microbiota, andFrontiers in microbiology · 2019Article
- Rhizosphere microbiome structure alters to enable wilt resistance in tomato.Nature biotechnology · 2018Article
- Discovery and control of culturable and viable but non-culturable cells of a distinctive Lactobacillus harbinensis strain from spoiled beer.Scientific reports · 2018Article
- Erwinia amylovora catalases KatA and KatG are virulence factors and delay the starvation-induced viable but non-culturable (VBNC) response.Molecular plant pathology · 2018Article
- Induction and Recovery of the Viable but Nonculturable State of Hop-ResistanceFrontiers in microbiology · 2018Article
- Persistence in Phytopathogenic Bacteria: Do We Know Enough?Frontiers in microbiology · 2018Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ralstonia solanacearum is the causal agent of bacterial wilt on a wide variety of plants, and enters a viable but nonculturable (VBNC) state under stress conditions in soil and water. Here, we adopted an artificial soil microcosm (ASM) to investigate the VBNC state of R. solanacearum induced by low temperature. The culturability of R. solanacearum strains SL341 and GMI1000 rapidly decreased at 4°C in modified ASM (mASM), while it was stably maintained at 25°C in mASM. We hypothesized that bacterial cells at 4°C in mASM are viable but nonculturable. Total protein profiles of SL341 cells at 4°C in mASM did not differ from those of SL341 culturable cells at 25°C in mASM. Moreover, the VBNC cells maintained in the mASM retained respiration activity. Catalase treatment effectively restored the culturability of nonculturable cells in mASM, while temperature increase or other treatments used for resuscitation of other bacteria were not effective. The resuscitated R. solanacearum from VBNC state displayed normal level of bacterial virulence on tomato plants compared with its original culturable bacteria. Expression of omp, oxyR, rpoS, dps, and the 16S rRNA gene quantified by RT-qPCR did not differ significantly between the culturable and VBNC states of R. solanacearum. Our results suggested that the VBNC bacterial cells in mASM induced by low temperature exist in a physiologically unique state.
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