ArticlePloS one2014
Identification and characterization of Wilt and salt stress-responsive microRNAs in chickpea through high-throughput sequencing.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers, 2 of them syntheses that pooled it.
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Who cites it
46 citing papers in PubMed, 2 syntheses or guidelines pooled it, 119 citations in OpenAlex.
- Developing resistance to Fusarium wilt in chickpea: From identifying meta-QTLs to molecular breeding.The plant genome · 2025Pooled it
- The role of microRNAs in responses to drought and heat stress in peanut (Arachis hypogaea).The plant genome · 2023Pooled it
- Association of uterine fibroids and pregnancy outcomes after ovarian stimulation-intrauterine insemination for unexplained infertility.Fertility and sterility · 2017Trial
- Comprehensive genome-wide analysis of DUF668 gene family in potato reveals roles in growth and stress responses.Journal of applied genetics · 2026Article
- Small but big player: the important role of microRNAs in legume crops.Molecular genetics and genomics : MGG · 2026Review
- Post-transcriptional and post-translational regulation of anthocyanin biosynthesis in sweetpotato by Ib-miR2111 and IbKFB: Implications for health promotion.Journal of advanced research · 2025Article
- Non-coding RNAs in plant stress responses: molecular insights and agricultural applications.Plant biotechnology journal · 2025Review
- Identification of miRNAs associated with Aspergillus flavus infection and their targets in groundnut (Arachis hypogaea L.).BMC plant biology · 2025Article
- Exploring the drought-responsive miRNAs and their corresponding target genes in chickpea root tissue.Plant molecular biology · 2025Article
- Non-coding RNAs and their role in plants: prospective omics-tools for improving growth, development and stress tolerance in field crops.Molecular biology reports · 2025Review
- Changes in epigenetic features in legumes under abiotic stresses.The plant genome · 2023Review
- Breeding and Genomic Approaches towards Development of Fusarium Wilt Resistance in Chickpea.Life (Basel, Switzerland) · 2023Review
- Shoot transcriptome revealed widespread differential expression and potential molecular mechanisms of chickpea (Frontiers in microbiology · 2023Article
- Genome-wide analysis and identification of microRNAs inFrontiers in plant science · 2023Article
- An atlas of small RNAs from potato.Plant direct · 2022Article
- Article
- Genome-wide identification and functional prediction of salt- stress related long non-coding RNAs (lncRNAs) in chickpea (Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- microRNA 166: an evolutionarily conserved stress biomarker in land plants targeting HD-ZIP family.Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Review
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Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chickpea (Cicer arietinum) is the second most widely grown legume worldwide and is the most important pulse crop in the Indian subcontinent. Chickpea productivity is adversely affected by a large number of biotic and abiotic stresses. MicroRNAs (miRNAs) have been implicated in the regulation of plant responses to several biotic and abiotic stresses. This study is the first attempt to identify chickpea miRNAs that are associated with biotic and abiotic stresses. The wilt infection that is caused by the fungus Fusarium oxysporum f.sp. ciceris is one of the major diseases severely affecting chickpea yields. Of late, increasing soil salinization has become a major problem in realizing these potential yields. Three chickpea libraries using fungal-infected, salt-treated and untreated seedlings were constructed and sequenced using next-generation sequencing technology. A total of 12,135,571 unique reads were obtained. In addition to 122 conserved miRNAs belonging to 25 different families, 59 novel miRNAs along with their star sequences were identified. Four legume-specific miRNAs, including miR5213, miR5232, miR2111 and miR2118, were found in all of the libraries. Poly(A)-based qRT-PCR (Quantitative real-time PCR) was used to validate eleven conserved and five novel miRNAs. miR530 was highly up regulated in response to fungal infection, which targets genes encoding zinc knuckle- and microtubule-associated proteins. Many miRNAs responded in a similar fashion under both biotic and abiotic stresses, indicating the existence of cross talk between the pathways that are involved in regulating these stresses. The potential target genes for the conserved and novel miRNAs were predicted based on sequence homologies. miR166 targets a HD-ZIPIII transcription factor and was validated by 5' RLM-RACE. This study has identified several conserved and novel miRNAs in the chickpea that are associated with gene regulation following exposure to wilt and salt stress.
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