ArticleIUCrJ2014
Room-temperature serial crystallography using a kinetically optimized microfluidic device for protein crystallization and on-chip X-ray diffraction.
Article in IUCrJ, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 49 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
49 citing papers in PubMed, 103 citations in OpenAlex.
- Thermally Regulated Droplet Microfluidics Enables Uniform, Size-Controllable DNA-Nanoparticle Superlattices.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Automated microbatch-under-oil phase diagrams to rationalize serial crystallography sample preparation.IUCrJ · 2026Article
- Microfluidic Devices for High-Throughput In Situ Serial Crystallography.Advances in biochemical engineering/biotechnology · 2026Review
- Target Engagement Assays in Early Drug Discovery.Journal of medicinal chemistry · 2025Review
- Review
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- JINXED: just in time crystallization for easy structure determination of biological macromolecules.IUCrJ · 2023Article
- Data collection from crystals grown in microfluidic droplets.Acta crystallographica. Section D, Structural biology · 2022Article
- Self-assembly of photonic crystals by controlling the nucleation and growth of DNA-coated colloids.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Nanocrystalline protein domains via salting-out.Acta crystallographica. Section F, Structural biology communications · 2021Article
- Novel combined crystallization plate for high-throughput crystal screening and in situ data collection at a crystallography beamline.Acta crystallographica. Section F, Structural biology communications · 2021Article
- A capillary-based microfluidic device enables primary high-throughput room-temperature crystallographic screening.Journal of applied crystallography · 2021Article
- Dynamic Structural Biology Experiments at XFEL or Synchrotron Sources.Methods in molecular biology (Clifton, N.J.) · 2021Review
- Room-temperature crystallography using a microfluidic protein crystal array device and its application to protein-ligand complex structure analysis.Chemical science · 2020Article
- Article
- Frontiers in Microfluidics, a Teaching Resource Review.Bioengineering (Basel, Switzerland) · 2019Review
- Cell-free expression of RNA encoded genes using MS2 replicase.Nucleic acids research · 2019Article
- 3D printed droplet generation devices for serial femtosecond crystallography enabled by surface coating.Journal of applied crystallography · 2019Article
- Design of Sealable Custom-Shaped Cell Mimicries Based on Self-Assembled Monolayers on CYTOP Polymer.ACS applied materials & interfaces · 2019Article
- Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
An emulsion-based serial crystallographic technology has been developed, in which nanolitre-sized droplets of protein solution are encapsulated in oil and stabilized by surfactant. Once the first crystal in a drop is nucleated, the small volume generates a negative feedback mechanism that lowers the supersaturation. This mechanism is exploited to produce one crystal per drop. Diffraction data are measured, one crystal at a time, from a series of room-temperature crystals stored on an X-ray semi-transparent microfluidic chip, and a 93% complete data set is obtained by merging single diffraction frames taken from different unoriented crystals. As proof of concept, the structure of glucose isomerase was solved to 2.1 Å, demonstrating the feasibility of high-throughput serial X-ray crystallography using synchrotron radiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.