ArticleFrontiers in microbiology2014
The stability region of the Streptomyces lividans plasmid pIJ101 encodes a DNA-binding protein recognizing a highly conserved short palindromic sequence motif.
Article in Frontiers in microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 11 citations in OpenAlex.
- Temperature-driven metabolic acceleration independent of cell growth enables high-level production of D-ribulose and shinorine in Streptomyces lividans.Biotechnology for biofuels and bioproducts · 2026Article
- Identification of plasmids from thermophilic Streptomyces strains and development of a gene cloning system for thermophilic Streptomyces species.Journal of industrial microbiology & biotechnology · 2024Article
- Engineering ofMolecules (Basel, Switzerland) · 2021Article
- Crosstalk between vertical and horizontal gene transfer: plasmid replication control by a conjugative relaxase.Nucleic acids research · 2017Article
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4 authors at 1 institution in 1 country.
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Abstract
Conjugation is a driving force in the evolution and shaping of bacterial genomes. In antibiotic producing streptomycetes even small plasmids replicating via the rolling-circle mechanism are conjugative. Although they encode only genes involved in replication and transfer, the molecular function of most plasmid encoded proteins is unknown. In this work we show that the conjugative plasmid pIJ101 encodes an overlooked protein, SpdA2. We show that SpdA2 is a DNA binding protein which specifically recognizes a palindromic DNA sequence (sps). sps is localized within the spdA2 coding region and highly conserved in many Streptomyces plasmids. Elimination of the palindrome or deletion of spdA2 in plasmid pIJ303 did not interfere with conjugative plasmid transfer or pock formation, but affected segregational stability.
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