ArticleDevelopment (Cambridge, England)2014
Subcellular localisations of the CPTI collection of YFP-tagged proteins in Drosophila embryos.
Article in Development (Cambridge, England), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed, 137 citations in OpenAlex.
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- A Notch signal required for a morphological novelty inScience advances · 2025Article
- Highly dynamic mechanical transitions in embryonic cell populations during Drosophila gastrulation.Nature communications · 2025Article
- The Drosophila epidermal growth factor receptor pathway regulates Hedgehog signalling and cytoneme behaviour.Nature communications · 2025Article
- Mechanical stress combines with planar polarised patterning during metaphase to orient embryonic epithelial cell divisions.Development (Cambridge, England) · 2024Article
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- Spatiotemporal remodeling of extracellular matrix orients epithelial sheet folding.Science advances · 2023Article
- Competition between myosin II and βeLife · 2023Article
- Novel roles for RNA binding proteins squid, hephaesteus, and Hrb27C in Drosophila oogenesis.Developmental dynamics : an official publication of the American Association of Anatomists · 2023Article
- The α-Catenin mechanosensing M region is required for cell adhesion during tissue morphogenesis.The Journal of cell biology · 2023Article
- Visualizing Fusome Morphology via Tubulin Immunofluorescence in Drosophila Ovarian Germ Cells.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Tricellulin secures the epithelial barrier at tricellular junctions by interacting with actomyosin.The Journal of cell biology · 2022Article
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4 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
A key challenge in the post-genomic area is to identify the function of the genes discovered, with many still uncharacterised in all metazoans. A first step is transcription pattern characterisation, for which we now have near whole-genome coverage in Drosophila. However, we have much more limited information about the expression and subcellular localisation of the corresponding proteins. The Cambridge Protein Trap Consortium generated, via piggyBac transposition, over 600 novel YFP-trap proteins tagging just under 400 Drosophila loci. Here, we characterise the subcellular localisations and expression patterns of these insertions, called the CPTI lines, in Drosophila embryos. We have systematically analysed subcellular localisations at cellularisation (stage 5) and recorded expression patterns at stage 5, at mid-embryogenesis (stage 11) and at late embryogenesis (stages 15-17). At stage 5, 31% of the nuclear lines (41) and 26% of the cytoplasmic lines (67) show discrete localisations that provide clues on the function of the protein and markers for organelles or regions, including nucleoli, the nuclear envelope, nuclear speckles, centrosomes, mitochondria, the endoplasmic reticulum, Golgi, lysosomes and peroxisomes. We characterised the membranous/cortical lines (102) throughout stage 5 to 10 during epithelial morphogenesis, documenting their apico-basal position and identifying those secreted in the extracellular space. We identified the tricellular vertices as a specialized membrane domain marked by the integral membrane protein Sidekick. Finally, we categorised the localisation of the membranous/cortical proteins during cytokinesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.