ArticleDevelopment (Cambridge, England)2014
Analysis of the expression patterns, subcellular localisations and interaction partners of Drosophila proteins using a pigP protein trap library.
Article in Development (Cambridge, England), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 109 papers.
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Who cites it
109 citing papers in PubMed.
- A natural programmable metamaterial controls 3D curvature of compound eyes.Nature communications · 2026Article
- Fasciclin 2 Cooperates with Discs Large to Maintain Epithelial Architecture.bioRxiv : the preprint server for biology · 2026Article
- Bridges under construction: the dynamics of ring canal expansion during Drosophila oogenesis.Developmental biology · 2026Review
- Imaging Techniques for the Study of Protein Condensates and Filaments and Their Applications.International journal of molecular sciences · 2026Review
- Matrix metalloproteinase 2 destabilizes Dally-like protein to restrict extracellular Wingless distribution.Molecular biology of the cell · 2025Article
- A Notch signal required for a morphological novelty inScience advances · 2025Article
- Highly dynamic mechanical transitions in embryonic cell populations during Drosophila gastrulation.Nature communications · 2025Article
- Clu1/Clu form mitochondria-associated granules upon metabolic transitions and regulate mitochondrial protein translation via ribosome interactions.PLoS genetics · 2025Article
- Single-cell analysis of the early Drosophila salivary gland reveals that morphogenetic control involves both the induction and exclusion of gene expression programs.PLoS biology · 2025Article
- Trithorax regulates long-term memory in Drosophila through epigenetic maintenance of mushroom body metabolic state and translation capacity.PLoS biology · 2025Article
- Molecular evidence supports the functionality of a protein-trapped endogenous allele of Dally-like protein.microPublication biology · 2025Article
- miRNA-mediated gene silencing in Drosophila larval development involves GW182-dependent and independent mechanisms.The EMBO journal · 2024Article
- Murine glial protrusion transcripts predict localized Drosophila glial mRNAs involved in plasticity.The Journal of cell biology · 2024Article
- A dystroglycan-laminin-integrin axis coordinates cell shape remodeling in the developing Drosophila retina.PLoS biology · 2024Article
- In vivo optogenetic manipulations of endogenous proteins reveal spatiotemporal roles of microtubule and kinesin in dendrite patterning.Science advances · 2024Article
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- Fas2EB112: a tale of two chromosomes.G3 (Bethesda, Md.) · 2024Article
- The Shot CH1 domain recognises a distinct form of F-actin during Drosophila oocyte determination.Development (Cambridge, England) · 2024Article
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49 more citing papers are in PubMed but not listed here.
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25 authors.
Funding
Abstract
Although we now have a wealth of information on the transcription patterns of all the genes in the Drosophila genome, much less is known about the properties of the encoded proteins. To provide information on the expression patterns and subcellular localisations of many proteins in parallel, we have performed a large-scale protein trap screen using a hybrid piggyBac vector carrying an artificial exon encoding yellow fluorescent protein (YFP) and protein affinity tags. From screening 41 million embryos, we recovered 616 verified independent YFP-positive lines representing protein traps in 374 genes, two-thirds of which had not been tagged in previous P element protein trap screens. Over 20 different research groups then characterized the expression patterns of the tagged proteins in a variety of tissues and at several developmental stages. In parallel, we purified many of the tagged proteins from embryos using the affinity tags and identified co-purifying proteins by mass spectrometry. The fly stocks are publicly available through the Kyoto Drosophila Genetics Resource Center. All our data are available via an open access database (Flannotator), which provides comprehensive information on the expression patterns, subcellular localisations and in vivo interaction partners of the trapped proteins. Our resource substantially increases the number of available protein traps in Drosophila and identifies new markers for cellular organelles and structures.
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