ArticleThe Journal of biological chemistry2014
Changes in transcription and metabolism during the early stage of replicative cellular senescence in budding yeast.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 46 citations in OpenAlex.
- Effects of micro- and nano-plastics exposure on cellular senescence: an overview.Archives of toxicology · 2026Review
- Sensitive, high-throughput, metabolic analysis by molecular sensors on the membrane surface of mother yeast cells.Nature communications · 2025Article
- Exploring the Role of Microplasma for Controlling Cellular Senescence inMolecules (Basel, Switzerland) · 2025Article
- Dynamic changes in gene expression through aging in Drosophila melanogaster heads.G3 (Bethesda, Md.) · 2025Article
- Dynamic Changes in Gene Expression Through Aging inbioRxiv : the preprint server for biology · 2024Article
- Millifluidic magnetophoresis-based chip for age-specific fractionation: evaluating the impact of age on metabolomics and gene expression in yeast.Lab on a chip · 2024Article
- Dietary change without caloric restriction maintains a youthful profile in ageing yeast.PLoS biology · 2023Article
- Multifarious Translational Regulation during Replicative Aging in Yeast.Journal of fungi (Basel, Switzerland) · 2022Article
- Recombinant protein linker production as a basis for non-invasive determination of single-cell yeast age in heterogeneous yeast populations.RSC advances · 2021Article
- Understanding the Impact of Industrial Stress Conditions on Replicative Aging inFrontiers in fungal biology · 2021Review
- Aspects of Multicellularity inGenes · 2020Review
- Trajectories of Aging: How Systems Biology in Yeast Can Illuminate Mechanisms of Personalized Aging.Proteomics · 2020Review
- Cell organelles and yeast longevity: an intertwined regulation.Current genetics · 2020Review
- Metabolic, Physiological, and Transcriptomics Analysis of Batch Cultures of the Green MicroalgaCells · 2019Article
- Proline metabolism regulates replicative lifespan in the yeastMicrobial cell (Graz, Austria) · 2019Article
- Transcriptomic and proteomic profiling revealed global changes in Streptococcus thermophilus during pH-controlled batch fermentations.Journal of microbiology (Seoul, Korea) · 2019Article
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- Gene expression hallmarks of cellular ageing.Biogerontology · 2018Review
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Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Age-related damage accumulates and a variety of biological activities and functions deteriorate in senescent cells. However, little is known about when cellular aging behaviors begin and what cellular aging processes change. Previous research demonstrated age-related mRNA changes in budding yeast by the 18th to 20th generation, which is the average replicative lifespan of yeast (i.e. about half of the population is dead by this time point). Here, we performed transcriptional and metabolic profiling for yeast at early stages of senescence (4th, 7th, and 11th generation), that is, for populations in which most cells are still alive. Transcriptional profiles showed up- and down-regulation for ∼20% of the genes profiled after the first four generations, few further changes by the 7th generation, and an additional 12% of the genes were up- and down-regulated after 11 generations. Pathway analysis revealed that these 11th generation cells had accumulated transcripts coding for enzymes involved in sugar metabolism, the TCA cycle, and amino acid degradation and showed decreased levels of mRNAs coding for enzymes involved in amino acid biosynthetic pathways. These observations were consistent with the metabolomic profiles of aging cells: an accumulation of pyruvic acid and TCA cycle intermediates and depletion of most amino acids, especially branched-chain amino acids. Stationary phase-induced genes were highly expressed after 11 generations even though the growth medium contained adequate levels of nutrients, indicating deterioration of the nutrient sensing and/or signaling pathways by the 11th generation. These changes are presumably early indications of replicative senescence.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.