ArticleNucleic acids research2014
Quantitative effect of target translation on small RNA efficacy reveals a novel mode of interaction.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- SUPER: Upcycling Genetic Parts for Precise Gene Expression Control, Leakage Minimization, and Genetic Circuit Stability.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Kinetic modeling reveals additional regulation at co-transcriptional level by post-transcriptional sRNA regulators.Cell reports · 2021Article
- Codon usage and modular interactions between messenger RNA coding regions and small RNAs in Escherichia coli.BMC genomics · 2018Article
- Dynamics of translation can determine the spatial organization of membrane-bound proteins and their mRNA.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Genetic variation in the 3'-untranslated region ofExperimental and therapeutic medicine · 2017Article
- Hierarchical Post-transcriptional Regulation of Colicin E2 Expression in Escherichia coli.PLoS computational biology · 2016Article
- Quantification of the gene silencing performances of rationally-designed synthetic small RNAs.Systems and synthetic biology · 2015Article
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Authors and funding
4 authors.
Funding
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Abstract
Small regulatory RNAs (sRNAs) in bacteria regulate many important cellular activities under normal conditions and in response to stress. Many sRNAs bind to the mRNA targets at or near the 5' untranslated region (UTR) resulting in translation inhibition and accelerated degradation. Often the sRNA-binding site is adjacent to or overlapping with the ribosomal binding site (RBS), suggesting a possible interplay between sRNA and ribosome binding. Here we combine quantitative experiments with mathematical modeling to reveal novel features of the interaction between small RNAs and the translation machinery at the 5'UTR of a target mRNA. By measuring the response of a library of reporter targets with varied RBSs, we find that increasing translation rate can lead to increased repression. Quantitative analysis of these data suggests a recruitment model, where bound ribosomes facilitate binding of the sRNA. We experimentally verified predictions of this model for the cell-to-cell variability of target expression. Our findings offer a framework for understanding sRNA silencing in the context of bacterial physiology.
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