ArticleNucleic acids research2014
A genome-wide function of THSC/TREX-2 at active genes prevents transcription-replication collisions.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 19 citations in OpenAlex.
- Point Mutations in the M Domain of PCID2 Impair Its Function in mRNA Export in Drosophila melanogaster.Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections · 2024Article
- Telomeric C-circles localize at nuclear pore complexes in Saccharomyces cerevisiae.The EMBO journal · 2022Article
- Stimulation of adaptive gene amplification by origin firing under replication fork constraint.Nucleic acids research · 2022Article
- THSC/TREX-2 deficiency causes replication stress and genome instability in Caenorhabditis elegans.Journal of cell science · 2021Article
- Review
- Nucleoporin TPR is an integral component of the TREX-2 mRNA export pathway.Nature communications · 2020Article
- Mip6 binds directly to the Mex67 UBA domain to maintain low levels of Msn2/4 stress-dependent mRNAs.EMBO reports · 2019Article
- The SAGA/TREX-2 subunit Sus1 binds widely to transcribed genes and affects mRNA turnover globally.Epigenetics & chromatin · 2018Article
- Review
- Mediator and TREX-2: Emerging links between transcription initiation and mRNA export.Nucleus (Austin, Tex.) · 2016Article
- Article
- Decoding mechanisms by which silent codon changes influence protein biogenesis and function.The international journal of biochemistry & cell biology · 2015Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The THSC/TREX-2 complex of Saccharomyces cerevisiae mediates the anchoring of transcribed genes to the nuclear pore, linking transcription elongation with mRNA export and genome stability, as shown for specific reporters. However, it is still unknown whether the function of TREX-2 is global and the reason for its relevant role in genome integrity. Here, by studying two TREX-2 representative subunits, Thp1 and Sac3, we show that TREX-2 has a genome-wide role in gene expression. Both proteins show similar distributions along the genome, with a gradient disposition at active genes that increases towards the 3' end. Thp1 and Sac3 have a relevant impact on the expression of long, G+C-rich and highly transcribed genes. Interestingly, replication impairment detected by the genome-wide accumulation of the replicative Rrm3 helicase is increased preferentially at highly expressed genes in the thp1Δ and sac3Δ mutants analyzed. Therefore, our work provides evidence of a function of TREX-2 at the genome-wide level and suggests a role for TREX-2 in preventing transcription-replication conflicts, as a source of genome instability derived from a defective messenger ribonucleoprotein particle (mRNP) biogenesis.
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