Evidence map›Paper›PMID 25293958›Full record

ArticleAngewandte Chemie (International ed. in English)2015

Multiple paramagnetic effects through a tagged reporter protein.

Aldo R Camacho-Zarco, Francesca Munari, Melanie Wegstroth, Wei-Min Liu, Marcellus Ubbink, Stefan Becker, Markus Zweckstetter

Open access · greenAbstract read
In one paragraph

Article in Angewandte Chemie (International ed. in English), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.3field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 18 citations in OpenAlex.

  1. A Two-Armed Probe for In-Cell DEER Measurements on Proteins*.Chemistry (Weinheim an der Bergstrasse, Germany) · 2020
    Article
  2. Article
  3. Review
  4. Article
  5. Review
  6. Paramagnetic Ligand Tagging To Identify Protein Binding Sites.Journal of the American Chemical Society · 2015
    Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Aldo R Camacho-ZarcoMax Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen (Germany); German Center for Neurodegenerative Diseases (DZNE), Göttingen (Germany); Center for the Molecular Physiology of the Brain, University Medical Center, Göttingen (Germany).
Francesca Munari
Melanie Wegstroth
Wei-Min Liu
Marcellus Ubbink
Stefan Becker
Markus Zweckstetter
Max Planck Institute for Biophysical Chemistry · DELeiden University · NLGerman Center for Neurodegenerative Diseases · DE

Funding

European Research Council 282008
6 · The paper itself

Abstract

Paramagnetic effects provide unique information about the structure and dynamics of biomolecules. We developed a method in which the lanthanoid tag is not directly attached to the protein of interest, but instead to a "reporter" protein, which binds and then transmits paramagnetic information to the target. The designed method allows access to a large number of paramagnetic restraints and residual dipolar couplings produced from independent molecular alignments in high-molecular-weight proteins with unknown 3D structure.

Indexed as

Nuclear Magnetic Resonance, BiomolecularAdaptor Proteins, Signal TransducingAmino Acid SequenceEscherichia coliHumansLanthanoid Series ElementsMaltose-Binding ProteinsModels, MolecularProteinsProtein Structure, TertiaryUbiquitinAdaptor Proteins, Signal TransducingLanthanoid Series ElementsMaltose-Binding ProteinsProteinsUbiquitinlanthanoidsNMR spectroscopyparamagnetismproteinsstructural biology

Identifiers

PMID25293958
PMCPMC5650053
OpenAlexW2141526139

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.