ArticleInsect molecular biology2015
Inducing RNA interference in the arbovirus vector, Culicoides sonorensis.
Article in Insect molecular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
- First molecular evidence of potential Culicoides vectors implicated in bluetongue virus transmission in Morocco.Parasites & vectors · 2024Article
- Article
- Outlook on RNAi-Based Strategies for ControllingPathogens (Basel, Switzerland) · 2023Review
- Evaluation of potential reference genes in the biting midge Culicoides sonorensis for real-time quantitative PCR analyses.Scientific reports · 2023Article
- microRNA Expression Dynamics inInsects · 2023Article
- Using Zoos as Sentinels for Re-Emerging Arboviruses: Vector Surveillance during an Outbreak of Epizootic Hemorrhagic Disease at the Minnesota Zoo.Pathogens (Basel, Switzerland) · 2023Article
- Modeling cellular co-infection and reassortment of bluetongue virus inVirus evolution · 2022Article
- Perspectives on the Changing Landscape of Epizootic Hemorrhagic Disease Virus Control.Viruses · 2021Review
- Next-generation tools to control biting midge populations and reduce pathogen transmission.Parasites & vectors · 2021Review
- An updated review on bluetongue virus: epidemiology, pathobiology, and advances in diagnosis and control with special reference to India.The veterinary quarterly · 2020Review
- The Genetic Diversification of a Single Bluetongue Virus Strain Using an In Vitro Model of Alternating-Host Transmission.Viruses · 2020Review
- The genome of the biting midge Culicoides sonorensis and gene expression analyses of vector competence for bluetongue virus.BMC genomics · 2018Article
- Functional Validation of Apoptosis Genes IAP1 and DRONC in Midgut Tissue of the Biting Midge Culicoides sonorensis (Diptera: Ceratopogonidae) by RNAi.Journal of medical entomology · 2017Article
- Bluetongue: control, surveillance and safe movement of animals.EFSA journal. European Food Safety Authority · 2017Article
- Dynamics of epizootic hemorrhagic disease virus infection within the vector, Culicoides sonorensis (Diptera: Ceratopogonidae).PloS one · 2017Article
- Non-structural protein NS3/NS3a is required for propagation of bluetongue virus in Culicoides sonorensis.Parasites & vectors · 2015Article
Corrections and comments
- Erratum issuedCorrigendum.2015
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Biting midges in the genus Culicoides are important vectors of arboviral diseases, including epizootic haemorrhagic disease, bluetongue and most likely Schmallenberg, which cause significant economic burdens worldwide. Research on these vectors has been hindered by the lack of a sequenced genome, the difficulty of consistent culturing of certain species and the absence of molecular techniques such as RNA interference (RNAi). Here, we report the establishment of RNAi as a research tool for the adult midge, Culicoides sonorensis. Based on previous research and transcriptome analysis, which revealed putative small interfering RNA pathway member orthologues, we hypothesized that adult C. sonorensis midges have the molecular machinery needed to perform RNA silencing. Injection of control double-stranded RNA targeting green fluorescent protein (dsGFP), into the haemocoel of 2-3-day-old adult female midges resulted in survival curves that support virus transmission. dsRNA injection targeting the newly identified C. sonorensis inhibitor of apoptosis protein 1 (CsIAP1) orthologue resulted in a 40% decrease of transcript levels and 73% shorter median survivals as compared with dsGFP-injected controls. These results reveal the conserved function of IAP1. Importantly, they also demonstrate the feasibility of RNAi by dsRNA injection in adult midges, which will greatly facilitate studies of the underlying mechanisms of vector competence in C. sonorensis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.