ArticlemBio2014
Bacterial nucleoid-associated protein uncouples transcription levels from transcription timing.
Article in mBio, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 32 citations in OpenAlex.
- Adaptation mechanisms of Brucella abortus to low magnesium ion stress.BMC veterinary research · 2025Article
- Multifaceted Evolution of the PhoPQ Two-Component System in Salmonella enterica Enhanced the Expression of Horizontally Acquired Virulence Genes.Molecular microbiology · 2025Article
- Coordination of Phosphate and Magnesium Metabolism in Bacteria.Advances in experimental medicine and biology · 2022Article
- How the PhoP/PhoQ System Controls Virulence and MgMicrobiology and molecular biology reviews : MMBR · 2021Review
- Quinazoline-Based Antivirulence Compounds Selectively TargetAntimicrobial agents and chemotherapy · 2019Article
- Article
- Overcoming the Cost of Positive Autoregulation by Accelerating the Response with a Coupled Negative Feedback.Cell reports · 2018Article
- Force-extension behavior of DNA in the presence of DNA-bending nucleoid associated proteins.The Journal of chemical physics · 2018Article
- Decoding a Salmonella Typhi Regulatory Network that Controls Typhoid Toxin Expression within Human Cells.Cell host & microbe · 2018Article
- Conformational dynamism for DNA interaction in the Salmonella RcsB response regulator.Nucleic acids research · 2018Article
- Quantitative Analysis of Intracellular Response Regulator Phosphatase Activity of Histidine Kinases.Methods in enzymology · 2018Article
- Control of virulence gene transcription by indirect readout in Vibrio cholerae and Salmonella enterica serovar Typhimurium.Environmental microbiology · 2017Review
- Article
- Spatial organization of DNA sequences directs the assembly of bacterial chromatin by a nucleoid-associated protein.The Journal of biological chemistry · 2017Article
- Facilitated Dissociation Kinetics of Dimeric Nucleoid-Associated Proteins Follow a Universal Curve.Biophysical journal · 2017Article
- Feedback Control of Two-Component Regulatory Systems.Annual review of microbiology · 2016Review
- Re-engineering cellular physiology by rewiring high-level global regulatory genes.Scientific reports · 2015Article
- Bacterial histone-like proteins: roles in stress resistance.Current genetics · 2015Review
- Regulation and function of the Salmonella MgtC virulence protein.Journal of microbiology (Seoul, Korea) · 2015Review
- When Too Much ATP Is Bad for Protein Synthesis.Journal of molecular biology · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
The histone-like nucleoid-structuring (H-NS) protein binds to horizontally acquired genes in the bacterium Salmonella enterica serovar Typhimurium, silencing their expression. We now report that overcoming the silencing effects of H-NS imposes a delay in the expression of genes activated by the transcriptional regulator PhoP. We determine that PhoP-activated genes ancestral to Salmonella are expressed before those acquired horizontally. This expression timing reflects the in vivo occupancy of the corresponding promoters by the PhoP protein. These results are surprising because some of these horizontally acquired genes reached higher mRNA levels than ancestral genes expressed earlier and were transcribed from promoters harboring PhoP-binding sites with higher in vitro affinity for the PhoP protein. Our findings challenge the often-made assumption that for genes coregulated by a given transcription factor, early genes are transcribed to higher mRNA levels than those transcribed at later times. Moreover, they provide a singular example of how gene ancestry can impact expression timing. Importance: We report that gene ancestry dictates the expression behavior of genes under the direct control of the Salmonella transcriptional regulator PhoP. That is, ancestral genes are transcribed before horizontally acquired genes. This reflects both the need to overcome silencing by the H-NS protein of the latter genes and the architecture of the corresponding promoters. Unexpectedly, transcription levels do not reflect transcription timing. Our results illustrate how a bacterium can exhibit an elaborate temporal expression behavior among genes coregulated by a transcription factor even though the products encoded by the target genes do not participate in a morphological or developmental pathway.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.