ArticleJournal of neuroscience methods2015
New methods for investigation of neuronal migration in embryonic brain explants.
Article in Journal of neuroscience methods, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- A Versatile Photocrosslinkable Silicone Composite for 3D Printing Applications.Advanced materials technologies · 2024Article
- An Interphase Microfluidic Culture System for the Study of Ex Vivo Intestinal Tissue.Micromachines · 2020Article
- Microfluidics for interrogating live intact tissues.Microsystems & nanoengineering · 2020Review
- Tissue Response to Neural Implants: The Use of Model Systems Toward New Design Solutions of Implantable Microelectrodes.Frontiers in neuroscience · 2019Review
- Ex Utero Electroporation and Organotypic Slice Cultures of Embryonic Mouse Brains for Live-Imaging of Migrating GABAergic Interneurons.Journal of visualized experiments : JoVE · 2018Article
- Cell Migration Research Based on Organ-on-Chip-Related Approaches.Micromachines · 2017Review
- Three-Dimensional Hyaluronic Acid Hydrogel-Based Models for In Vitro Human iPSC-Derived NPC Culture and Differentiation.Journal of materials chemistry. B · 2017Article
- In Vitro, Ex Vivo and In Vivo Techniques to Study Neuronal Migration in the Developing Cerebral Cortex.Brain sciences · 2017Review
- Biology-inspired microphysiological system approaches to solve the prediction dilemma of substance testing.ALTEX · 2016Review
- Microfluidic Organ/Body-on-a-Chip Devices at the Convergence of Biology and Microengineering.Sensors (Basel, Switzerland) · 2015Review
- Organ-on-a-Chip: New Platform for Biological Analysis.Analytical chemistry insights · 2015Review
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Authors and funding
8 authors.
Funding
Abstract
backgroundProper migration of neurons is essential for the formation and normal functioning of the nervous system. Defects in neuronal migration underlie a number of neurologic diseases in humans. Although cell migration is crucial for neural development, molecular mechanisms guiding neuronal migration remain to be elucidated fully. Newborn neurons from the embryonic medial ganglionic eminence (MGE) migrate a long distance dorsally in the developing brain, giving rise to several types of interneurons in the neocortex. NEW
methodIn this study, we developed an immunocytochemistry (ICC) protocol to stain neurons migrating out of the MGE explant embedded in Matrigel. We also established a protocol to efficiently transfect cells in MGE explants, achieving a transduction efficiency of more than 30%. COMPARISON WITH EXISTING
methodIn addition, we developed microfluidic chambers for explants that allow visualization of the vectorial migration of individual neurons from mouse embryonic MGE explants. Our microfluidic system allows monitoring of the distribution of cellular organelles (e.g. Golgi) within migrating neurons which have been stained with commercial molecular dyes or transfected with adeno-associated virus (AAV) expressing reporter proteins.
conclusionThese methods provide new paradigms to study neuronal migration in real-time.
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