ArticleNature communications2014
Proof of dual-topology architecture of Fluc F- channels with monobody blockers.
Article in Nature communications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 55 citations in OpenAlex.
- Monovalent cations differentially regulate the activity of eukaryotic fluoride channels and impact fluoride resistance.PLoS biology · 2026Article
- Asymmetry-induced distinct mechanisms and the transporting role of sodium in bacterial fluoride channel Fluc.bioRxiv : the preprint server for biology · 2026Article
- The molecular mechanism of fluoride export by the eukaryotic fluoride channel FEX.Nature communications · 2025Article
- Molecular Insights into Fluoride Ion Uptake and Selectivity in the CLCF Fluoride/Proton Antiporter.The journal of physical chemistry. B · 2025Article
- The link between ancient microbial fluoride resistance mechanisms and bioengineering organofluorine degradation or synthesis.Nature communications · 2024Review
- Toward the development of a molecular toolkit for the microbial remediation of per-and polyfluoroalkyl substances.Applied and environmental microbiology · 2024Review
- Lanthanum-fluoride electrode-based methods to monitor fluoride transport in cells and reconstituted lipid vesicles.Methods in enzymology · 2024Article
- Fluoride permeation mechanism of the Fluc channel in liposomes revealed by solid-state NMR.Science advances · 2023Article
- Controlling ion channel function with renewable recombinant antibodies.The Journal of physiology · 2022Review
- Ion permeation, selectivity, and electronic polarization in fluoride channels.Biophysical journal · 2022Article
- Article
- Membrane Exporters of Fluoride Ion.Annual review of biochemistry · 2021Review
- Structure and dynamics of the drug-bound bacterial transporter EmrE in lipid bilayers.Nature communications · 2021Article
- The structural basis of promiscuity in small multidrug resistance transporters.Nature communications · 2020Article
- An Interfacial Sodium Ion is an Essential Structural Feature of Fluc Family Fluoride Channels.Journal of molecular biology · 2020Article
- Transmembrane Fluoride Transport by a Cyclic Azapeptide With Two β-Turns.Frontiers in chemistry · 2020Article
- Nitrate and Phosphate Transporters Rescue Fluoride Toxicity in Yeast.Chemical research in toxicology · 2019Article
- Fast and selective fluoride ion conduction in sub-1-nanometer metal-organic framework channels.Nature communications · 2019Article
- Antibodies and venom peptides: new modalities for ion channels.Nature reviews. Drug discovery · 2019Review
- Synthetic single domain antibodies for the conformational trapping of membrane proteins.eLife · 2018Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Fluc-type F(-) channels--used by microorganisms for resisting fluoride toxicity--are unusual in their quaternary architecture: they are thought to associate as dimers with the two subunits in antiparallel transmembrane orientation. Here, we subject this unusual structural feature to a direct test. Single purified Fluc channels recorded in planar lipid bilayers are constitutively open, with rare, short-lived closings. Using combinatorial libraries, we generated synthetic binding proteins, 'monobodies,' that specifically bind to Fluc homologues with nanomolar affinity. Reversible binding of monobodies to two different Fluc channel homologues is seen in single-channel recordings as long-lived nonconducting events that follow bimolecular kinetics. By applying monobodies sequentially to the two sides of the bilayer in a double-sided perfusion manoeuvre, we show that Fluc channels present monobody-binding epitopes to both sides of the membrane. The result establishes that Fluc subunits are arranged in dimeric antiparallel orientation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.