Evidence map›Paper›PMID 25289325›Full record

ArticlePlastic and reconstructive surgery. Global open2014

Overexpressed HIF-2α in Endothelial Cells Promotes Vascularization and Improves Random Pattern Skin Flap Survival.

Atsushi Morimoto, Shuhei Tomita, Masaki Imanishi, Go Shioi, Yoshitaka Kihira, Yuki Izawa-Ishizawa, Mitsuru Takaku, Ichiro Hashimoto, Yasumasa Ikeda, Hideki Nakanishi and 1 more

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Article in Plastic and reconstructive surgery. Global open, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Atsushi MorimotoDepartment of Plastic and Reconstructive Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, Kuramoto, Tokushima, Japan; Division of Molecular Pharmacology, Tottori University Faculty of Medicine, Nishi-cho, Yonago, Japan; Department of Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School, Kuramoto, Tokushima, Japan; and Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology, Chuou-ku, Kobe, Japan.
Shuhei TomitaDepartment of Plastic and Reconstructive Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, Kuramoto, Tokushima, Japan; Division of Molecular Pharmacology, Tottori University Faculty of Medicine, Nishi-cho, Yonago, Japan; Department of Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School, Kuramoto, Tokushima, Japan; and Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology, Chuou-ku, Kobe, Japan.
Masaki ImanishiDepartment of Plastic and Reconstructive Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, Kuramoto, Tokushima, Japan; Division of Molecular Pharmacology, Tottori University Faculty of Medicine, Nishi-cho, Yonago, Japan; Department of Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School, Kuramoto, Tokushima, Japan; and Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology, Chuou-ku, Kobe, Japan.
Go ShioiDepartment of Plastic and Reconstructive Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, Kuramoto, Tokushima, Japan; Division of Molecular Pharmacology, Tottori University Faculty of Medicine, Nishi-cho, Yonago, Japan; Department of Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School, Kuramoto, Tokushima, Japan; and Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology, Chuou-ku, Kobe, Japan.
Yoshitaka KihiraDepartment of Plastic and Reconstructive Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, Kuramoto, Tokushima, Japan; Division of Molecular Pharmacology, Tottori University Faculty of Medicine, Nishi-cho, Yonago, Japan; Department of Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School, Kuramoto, Tokushima, Japan; and Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology, Chuou-ku, Kobe, Japan.
Yuki Izawa-IshizawaDepartment of Plastic and Reconstructive Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, Kuramoto, Tokushima, Japan; Division of Molecular Pharmacology, Tottori University Faculty of Medicine, Nishi-cho, Yonago, Japan; Department of Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School, Kuramoto, Tokushima, Japan; and Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology, Chuou-ku, Kobe, Japan.
Mitsuru TakakuDepartment of Plastic and Reconstructive Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, Kuramoto, Tokushima, Japan; Division of Molecular Pharmacology, Tottori University Faculty of Medicine, Nishi-cho, Yonago, Japan; Department of Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School, Kuramoto, Tokushima, Japan; and Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology, Chuou-ku, Kobe, Japan.
Ichiro HashimotoDepartment of Plastic and Reconstructive Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, Kuramoto, Tokushima, Japan; Division of Molecular Pharmacology, Tottori University Faculty of Medicine, Nishi-cho, Yonago, Japan; Department of Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School, Kuramoto, Tokushima, Japan; and Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology, Chuou-ku, Kobe, Japan.
Yasumasa IkedaDepartment of Plastic and Reconstructive Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, Kuramoto, Tokushima, Japan; Division of Molecular Pharmacology, Tottori University Faculty of Medicine, Nishi-cho, Yonago, Japan; Department of Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School, Kuramoto, Tokushima, Japan; and Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology, Chuou-ku, Kobe, Japan.
Hideki NakanishiDepartment of Plastic and Reconstructive Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, Kuramoto, Tokushima, Japan; Division of Molecular Pharmacology, Tottori University Faculty of Medicine, Nishi-cho, Yonago, Japan; Department of Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School, Kuramoto, Tokushima, Japan; and Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology, Chuou-ku, Kobe, Japan.
Toshiaki TamakiDepartment of Plastic and Reconstructive Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, Kuramoto, Tokushima, Japan; Division of Molecular Pharmacology, Tottori University Faculty of Medicine, Nishi-cho, Yonago, Japan; Department of Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School, Kuramoto, Tokushima, Japan; and Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology, Chuou-ku, Kobe, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe local skin flap procedure is very useful for reconstruction. However, flap necrosis caused by circulatory failure can occur at its distal portion. Hypoxia-inducible factors (HIFs) in endothelial cells (ECs) help to maintain ECs and promote vascularization, and HIF-2α is abundantly expressed in ECs. However, the mechanisms of action of HIF-2α in ECs are not yet fully understood. The aim of this study was to evaluate the in vivo effects of overexpression of HIF-2α in ECs on skin flap survival.

methodsA random pattern skin flap (1.0 × 3.0 cm) was elevated on the dorsum of transgenic mice (Tg mice) with EC-specific HIF-2α conditional overexpression and wild-type littermate control mice (n = 6). Flap survival was evaluated on postoperative day 7. Tissue samples from the skin flaps were harvested and analyzed using Western blotting, quantitative reverse transcriptase-polymerase chain reaction, and immunohistochemistry.

resultsThe HIF-2α mRNA and protein levels were significantly increased in the Tg mice when compared with control mice. Tg mice had significantly increased skin flap survival areas (72.0% ± 2.7%) when compared with wild-type mice (45.7% ± 1.1%). Moreover, histological examination revealed an increase in the subcutaneous blood vessel counts in the Tg mice.

conclusionsSpecific overexpression of HIF-2α in ECs promoted vascularization and enhanced skin flap survival in vivo in a mouse model.

Identifiers

PMID25289325
PMCPMC4174206

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