Evidence map›Paper›PMID 25289028›Full record

ArticleExperimental and therapeutic medicine2014

Roles of the co-culture of human umbilical cord Wharton's jelly-derived mesenchymal stem cells with rat pancreatic cells in the treatment of rats with diabetes mellitus.

Guangyu Wang, Yong Li, Yu Wang, Yu Dong, Fu-Sheng Wang, Yi Ding, Yudong Kang, Xuying Xu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental and therapeutic medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Guangyu WangUlcerous Vascular Surgical Department, Beijing TCM Hospital Affiliated to Capital Medical University, Beijing 100010, P.R. China.
Yong LiUlcerous Vascular Surgical Department, Beijing TCM Hospital Affiliated to Capital Medical University, Beijing 100010, P.R. China.
Yu WangUlcerous Vascular Surgical Department, Beijing TCM Hospital Affiliated to Capital Medical University, Beijing 100010, P.R. China.
Yu DongUlcerous Vascular Surgical Department, Beijing TCM Hospital Affiliated to Capital Medical University, Beijing 100010, P.R. China.
Fu-Sheng WangUlcerous Vascular Surgical Department, Beijing TCM Hospital Affiliated to Capital Medical University, Beijing 100010, P.R. China.
Yi DingUlcerous Vascular Surgical Department, Beijing TCM Hospital Affiliated to Capital Medical University, Beijing 100010, P.R. China.
Yudong KangUlcerous Vascular Surgical Department, Beijing TCM Hospital Affiliated to Capital Medical University, Beijing 100010, P.R. China.
Xuying XuUlcerous Vascular Surgical Department, Beijing TCM Hospital Affiliated to Capital Medical University, Beijing 100010, P.R. China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The aim of the present study was to investigate the roles of the co-culture of human umbilical cord Wharton's jelly-derived mesenchymal stem cells (hUC-MSCs) with rat pancreatic cells in the treatment of rats with diabetes mellitus. hUC-MSCs were isolated and passaged, followed by Transwell co-culture with rat pancreatic cells. The induced islet-like cell clusters were transplanted into the renal capsule in rats with streptozotocin (STZ)-induced diabetes mellitus. The effects of co-culture on blood glucose levels in rats were observed. The isolated hUC-MSCs expressed the specific surface markers, including cluster of differentiation 44 (CD44) (91.4%), CD29 (91.3%) and CD105 (99.2%). Following co-culture with hUC-MSCs for 7 and 10 days, the rat pancreatic cells were strongly stained by pancreatic and duodenal homeobox-1 and human insulin. The insulin and C-peptide concentrations were increased significantly compared to the pure culture group. One week following the transplantation of induced islet-like cells into the renal capsule, the blood glucose level of rats in the STZ experimental group was significantly lower than that of the STZ control group. There were notable 5-bromo-2'-deoxyuridine-positive nuclei and insulin-positive cytoplasm in the renal capsule following cell transplantation. Therefore, co-culture of hUC-MSCs with rat pancreatic cells can lower the blood glucose levels in rats with diabetes mellitus.

Indexed as

diabetes mellitusmesenchymal stem cellstransplantationumbilical cordWharton’s jelly

Identifiers

PMID25289028
PMCPMC4186331

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.