ArticleThe plant pathology journal2013
Sampling and Selection Factors that Enhance the Diversity of Microbial Collections: Application to Biopesticide Development.
Article in The plant pathology journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 9 citations in OpenAlex.
- Spatial and Temporal Variation of Cultivable Communities of Co-occurring Endophytes and Pathogens in Wheat.Frontiers in microbiology · 2016Article
- What's Inside That Seed We Brew? A New Approach To Mining the Coffee Microbiome.Applied and environmental microbiology · 2015Review
- Association of shifting populations in the root zone microbiome of millet with enhanced crop productivity in the Sahel region (Africa).Applied and environmental microbiology · 2015Article
- Development of a freeze-dried fungal wettable powder preparation able to biodegrade chlorpyrifos on vegetables.PloS one · 2014Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Diverse bacteria are known to colonize plants. However, only a small fraction of that diversity has been evaluated for their biopesticide potential. To date, the criteria for sampling and selection in such bioprospecting endeavors have not been systematically evaluated in terms of the relative amount of diversity they provide for analysis. The present study aimed to enhance the success of bio-prospecting efforts by increasing the diversity while removing the genotypic redundancy often present in large collections of bacteria. We developed a multivariate sampling and marker-based selection strategy that significantly increase the diversity of bacteria recovered from plants. In doing so, we quantified the effects of varying sampling intensity, media composition, incubation conditions, plant species, and soil source on the diversity of recovered isolates. Subsequent sequencing and high-throughput phenotypic analyses of a small fraction of the collected isolates revealed that this approach led to the recovery of over a dozen rare and, to date, poorly characterized genera of plant-associated bacteria with significant biopesticide activities. Overall, the sampling and selection approach described led to an approximately 5-fold improvement in efficiency and the recovery of several novel strains of bacteria with significant biopesticide potential.
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Registered trials
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